MSDS Cryopreserved Cells
Instructions HCEpC
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Description
Human Corneal Epithelial Cells (HCEpC) are isolated from the epithelium of the Human Cornea. They are cryopreserved at the second passage and be cultured and propagated for at least 12 population doublings. These cells have been used as models for studying the re-epithelialization of the eye following photorefractive keratectomy, and neutrophil induced corneal damage. They have also been used to study the activity of Matrix Metalloproteinases in the Corneal Epithelium. Human Corneal Epithelial Cells can also be used in research aimed at reconstructing damaged corneas.
(Click to Enlarge) Human Corneal Epithelial Cells (HCEpC). Phase-contrast (Top) and fluorescent (Bottom) images stained with CK18 and E-Cadherin.
Details
Tissue | Normal healthy human cornea |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Bioassay | Attach, spread, proliferate in Growth Med |
Cryovial | 500,000 HCEpC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
Kit | Cryovial frozen HCEpC, Growth Medium (211-500), Subculture Rgnt Kit (090K) |
Proliferating | Shipped in Tsfr Med, psg 1, flasks or plates |
| Doublings | At least 12 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Corneal Epithelial Cells Total Kit, adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 630K-05a | Price: $903.00 | |
| Cryopreserved Corneal Epithelial Cells (HCEpC), adult: Frozen HCEpC (5x10^5) | Size: 1 Cryovial | CAT.#: 630-05a | Price: $715.00 | |
| Proliferating Corneal Epithelial Cells (HCEpC), adult: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 631-25a | Price: $715.00 | |
| Proliferating Corneal Epithelial Cells (HCEpC), adult: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 631-75a | Price: $905.00 | |
| Proliferating Corneal Epithelial Cells (HCEpC), adult: Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: 631-24Wa | Price: $905.00 | |
| Proliferating Corneal Epithelial Cells (HCEpC), adult: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 631-96Wa | Price: $1,025.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| HCEpC Growth Medium: "Corne-Epi" all-in-one ready-to-use Medium, contains BPE but no FBS | Size: 500 ml | CAT.#: 221-500 | Price: $135.00 | |
| HCEpC Growth Medium Kit: "Corne-Epi" basal medium & growth supplement sold together packaged separately, contains BPE but no FBS | Size: 500 ml | CAT.#: 221K-500 | Price: $145.00 | |
| HCEpC Basal Medium: "Corne-Epi" basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 220-500 | Price: $81.00 | |
| HCEpC Growth Supplement: "Corne-Epi" GS, added to Basal Medium to create Growth Medium, contains BPE but no FBS | Size: 5 ml (3 parts) | CAT.#: 221-GS | Price: $86.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Monoclonal IL-1 Receptor Antagonist Antibody: Monoclonal IL-1 Receptor Antagonist Antibody | Size: 100 ul | CAT.#: CB19846 | Price: $302.00 | |
| Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. | Size: 50 ml | CAT.#: 040-50 | Price: $54.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Cytofect Epithelial Cell Transfection Kit (124 x 24-Wells): 124 x 24-Well Rxns | Size: 1 Kit | CAT.#: TF102K | Price: $465.00 | |
| Cytofect Epithelial Cell Transfection Sample Kit (25 x 24-Wells): 25 x 24-Well Rxns | Size: 1 Sample Kit | CAT.#: TF102KS | Price: $62.00 | |
| Human IL-1 alpha ELISA Kit: Human Interleukin-1 alpha ELISA Kit | Size: 96 wells | CAT.#: CL0389 | Price: $484.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 10 ug | CAT.#: RP1173-10 | Price: $194.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 100 ug | CAT.#: RP1173-100 | Price: $624.00 | |
| Human Interleukin-1-Alpha (IL-1 alpha): Human Interleukin-1-alpha | Size: 1000 ug | CAT.#: RP1173-1000 | Price: $5,327.00 | |
| Human IL-1 beta ELISA Kit: Human Interleukin-1 beta ELISA Kit | Size: 96 wells | CAT.#: CL0392 | Price: $587.00 | |
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 |