MSDS Cryopreserved Cells
Instructions PCASMC
Cell Apps Flyer Smooth Muscle Cells
Cell Apps Poster Primary Cells
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Applications Inc Brochure

Description

Porcine Coronary Artery Smooth Muscle Cells (PCASMC) from Cell Applications, Inc. were used to show that:
  • c-Jun plays a role in smooth muscle proliferation
  • Inhibition of c-Jun abrogates smooth muscle cell growth and intimal thickening after arterial injury
  • Nanofibrous scaffold material has potential for tissue engineering
Characterization: positive for smooth muscle cell specific alpha-actin expression

(Click to Enlarge) Porcine Coronary Artery Smooth Muscle Cells: PCASMC (L), immunolabeled for smooth muscle-specific alpha-actin using the ABC method (R).

Details

Tissue:
Normal healthy porcine coronary artery
QC
No bacteria, yeast, fungi, mycoplasma
Character
Smooth muscle specific α-actin positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 PCASMC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen PCASMC (P350-05), Grwth Medium (P311-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 16
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions PCASMC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved PCASMC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: P350K-05 Price: $769.00
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Cryopreserved PCASMC: Frozen PCASMC (5x10^5) Size: 1 Cryovial CAT.#: P350-05 Price: $619.00
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Proliferating PCASMC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: P351-25 Price: $619.00
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Proliferating PCASMC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: P351-75 Price: $823.00
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Proliferating PCASMC: Actively growing, dividing cells in medium Size: 6 Well CAT.#: P351-6W Price: $823.00
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Proliferating PCASMC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: P351-96W Price: $952.00
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Related Products

Product Size CAT.# Price Quantity
Porcine SMC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: P310-500 Price: $57.00
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Porcine SMC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: P311-500 Price: $105.00
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Porcine SMC Growth Supplement: Added to Basal Growth Medium to create Growth Medium Size: 50 ml CAT.#: P311-GS Price: $57.00
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Porcine SMC Induction Medium: Provides signals that change cell behavior Size: 250 ml CAT.#: P311I-250 Price: $62.00
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Extended Family Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 125 x 24-Well Size: 1 Kit CAT.#: TF350K Price: $285.00
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Cytofect™ Smooth Muscle Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF350KS Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Smooth Muscle Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2012
Khachigian, L. 2012. Vascular therapeutics. Patent US 8242090 B2.
2009
Yixiang, D. 2009. Nanofiber Covered Stent for Vascular Diseases. Thesis, National University of Singapore.
2008
Dong, Y., T. Yong, S. Liao, C.K. Chan, and S. Ramakrishna. 2008. Long-term viability of coronary artery smooth muscle cells on poly (l-lactide-co-ϵ-caprolactone) nanofibrous scaffold indicates its potential for blood vessel tissue engineering. Journal of The Royal Society Interface. 5:1109-1118.
2002
Khachigian, L.M., R.G. Fahmy, G. Zhang, Y.V. Bobryshev, and A. Kaniaros. 2002. c-Jun Regulates Vascular Smooth Muscle Cell Growth and Neointima Formation after Arterial Injury. Journal of Biological Chemistry. 277:22985-22991.