MSDS Cryopreserved Cells
Instructions PCAEC
5 Important Cell Culture Rules
Cell Apps Fllyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Porcine Coronary Artery Endothelial Cells (PCAEC) provide a useful tool to study artery endothelial function and metabolism.  Arterial endothelial cells play critical roles in cardiac homeostasis, and insults to the endothelial cells have been linked to a variety of vascular diseases.  
PCAEC from Cell Applications, Inc. have been used to:
  • Investigate a new mechanism of obesity-associated vascular disease by demonstrating that resistin, an adipocyte-derived cytokine implemented in insulin resistance and diabetes, can cause endothelial dysfunction in coronary arteries through oxidative stress and down-regulation of NO synthase
  • Show that secretoneurin activates expression of NO synthase and calmodulin, leading to endothelial relaxation
  • Reveal anti-inflammatory, cardioprotective effects of a novel adenosine-lidocaine drug formulation by observing adhesion and transmigration
(Click to Enlarge) Porcine Coronary Artery Endothelial: PCAEC

Details

Tissue
Normal healthy porcine coronary artery
QC
No bacteria, yeast, fungi, mycoplasma
Character
DiI-Ac-LDL uptake: Positive
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 PAOEC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen PCAEC (P300-05), Growth Medium (P211-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 12
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions PCAEC

Format: PDF

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved PCAEC: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: P300K-05 Price: $780.00
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Cryopreserved PCAEC: Frozen PCAEC (5x10^5) Size: 1 Cryovial CAT.#: P300-05 Price: $630.00
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Proliferating PCAEC: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: P301-25 Price: $630.00
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Proliferating PCAEC: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: P301-75 Price: $834.00
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Proliferating PCAEC: Actively growing, dividing cells in medium Size: 24 Well CAT.#: P301-24W Price: $834.00
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Proliferating PCAEC: Actively growing, dividing cells in medium Size: 96 Well CAT.#: P301-96W Price: $963.00
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Related Products

Product Size CAT.# Price Quantity
Porcine EC Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: P210-500 Price: $54.00
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Porcine EC Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: P211-500 Price: $105.00
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Porcine EC Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: P211K-500 Price: $113.00
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Porcine EC Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: P211-GS Price: $57.00
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Extended Family Products

Product Size CAT.# Price Quantity
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $431.00
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $54.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2012
Shi, W., R. Jiang, G.P. Dobson, A. Granfeldt, and J. Vinten-Johansen. 2012. The nondepolarizing, normokalemic cardioplegia formulation adenosine-lidocaine (adenocaine) exerts anti-neutrophil effects by synergistic actions of its components. The Journal of Thoracic and Cardiovascular Surgery. 143:1167-1175.
2011
han, C.K., and P.M. Vanhoutte. 2011. Secretoneurin facilitates endothelium-dependent relaxations in porcine coronary arteries. American Journal of Physiology-Heart and Circulatory Physiology. 300:H1159-H1165.
2006
Zhou, W., H. Chai, A. Courson, P. Lin, A. Lumsden, Q. Yao, and C. Chen. 2006. Ginkgolide A attenuates homocysteine-induced endothelial dysfunction in porcine coronary arteries. J Vascular Surg, 44:853-862.
2005

Kougias, P., H. Chai, P.H. Lin, A.B. Lumsden, Q. Yao, and C. Chen. 2005. Adipocyte-derived cytokine resistin causes endothelial dysfunction of porcine coronary arteries. Journal of Vascular Surgery. 41:691-698.