MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Cell Apps Fllyer Cardiovascular Cells
Instructions PCVIC
Description
Porcine Cardiac Valve Interstitial Cells (PCVIC) are a heterogeneous population of specific cell types responsible for maintaining the structural integrity and normal functioning of the valve. Primary culture of valve interstitial cells is useful in exploring cellular and molecular pathogenesis of heart valve disease and discovering new therapeutic targets for cardiovascular diseases.
PCVIC from Cell Applications, Inc. are derived from porcine heart and cultured in Porcine Cardiac Valve Interstitial Cell Growth Medium. PCVIC can propagate to 10 population doublings when cultured under the recommended conditions and provide an excellent model system to study heart valve function and pathobiology.
Characterization: Positive for α-smooth muscle actin and vimentin by ICC staining.
Top: Porcine Cardiac Valve Interstitial Cells (PCVIC). Bottom left: PCVIC stained for vimentin (in green) and DAPI (in blue). Bottom right: PCVIC stained for α-smooth muscle actin (in green) and DAPI (in blue).
Details
| Tissue | Normal healthy porcine heart |
| QC | No bacteria, yeast, fungi, mycoplasma |
| Character | Positive for α-smooth muscle actin and vimentin |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 PCVIC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen PCVIC (P370-05n), Growth Medium (P3113-500), Subculture Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
| Doublings | At least 15 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Porcine Cardiac Valve Interstitial Cells Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: P370K-05 | Price: $960.00 | |
| Cryopreserved Porcine Cardiac Valve Interstitial Cells (PCVIC): Frozen PCVIC (5x10^5) | Size: 1 Cryovial | CAT.#: P370-05 | Price: $775.00 | |
| Proliferating Porcine Cardiac Valve Interstitial Cells (PCVIC): Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: P371-25 | Price: $775.00 | |
| Proliferating Porcine Cardiac Valve Interstitial Cells (PCVIC): Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: P371-75 | Price: $965.00 | |
| Proliferating Porcine Cardiac Valve Interstitial Cells (PCVIC): Actively growing, dividing cells in medium | Size: 24 Well | CAT.#: P371-24W | Price: $965.00 | |
| Proliferating Porcine Cardiac Valve Interstitial Cells (PCVIC): Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: P371-96W | Price: $1,085.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Porcine Cardiac Valve Interstitial Cells Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: P3113-500 | Price: $132.00 | |
| Porcine Cardiac Valve Interstitial Cells Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: P3113K-500 | Price: $144.00 | |
| Porcine Cardiac Valve Interstitial Cells Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 30 ml | CAT.#: P3113-GS | Price: $79.00 | |
| Porcine Cardiac Valve Interstitial Cells Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: P3112-500 | Price: $70.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 |