MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions MHSC

Description

Mouse Hepatic Stellate Cells (MHSC) are one of the important cell type in the liver. Hepatic Stellate Cells play an important role in liver homeostasis, maintenance and regeneration including retinol metabolism. Activation of stellate cells in disease state leads to liver fibrosis, hepatitis B & C, fatty liver disease, diabetes and other chronic diseases.
 
Hepatic Stellate Cells are primary cells derived from human liver and cultured in optimized Hepatic Stellate Cells Growth Medium. MHSCs from Cell Applications, Inc. provide an excellent model system to study many aspects of human liver function, metabolism and pathophysiology. Primary culture of Hepatic Stellate Cells is suitable for many cell-based assays including toxicity, drug screening and metabolism and is a valuable tool to study liver function, physiology and liver diseases.
 
Characterization: Positive for α-smooth muscle actin by ICC staining.
Mouse Hepatic Stellate Cells: MHSC Left: phase contrast, Right: HHF immunolabeled for α-smooth muscle actin (green), nuclei are stained with DAPI.

Details

TissueNormal healthy mouse liver tissue
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 MHSC (3rd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
KitCryovial frozen MHSC (M782-05a), Growth Medium (M7117-500), Subcltr Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 3rd psg (flasks or plates)
DoublingsAt least 10
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

Instructions MHSC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved MHSC, adult: Frozen MHSC (5x10^5) Size: 1 Cryovial CAT.#: M782-05a Price: $425.00
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Cryopreserved MHSC, Adult: Cryopreserved MHSC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: M782K-05a Price: $595.00
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Proliferating MHSC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: M783-25a Price: $425.00
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Proliferating MHSC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: M783-75a Price: $615.00
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Proliferating MHSC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: M783-6Wa Price: $615.00
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Proliferating MHSC, adult:  Actively growing, dividing cells in medium Size: 96 Well CAT.#: M783-96Wa Price: $735.00
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Related Products

Product Size CAT.# Price Quantity
Mouse Hepatic Stellate Cells Basal Medium: Basal medium (contains no growth supplement). Add GS before use. Size: 500 ml CAT.#: M7116-500 Price: $57.00
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Mouse Hepatic Stellate Cells Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: M7117-500 Price: $125.00
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Mouse Hepatic Stellate Cells Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: M7117K-500 Price: $135.00
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Mouse Hepatic Stellate Cells Growth Medium Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: M7117-GS Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now