Instructions MCoN M822N-20
Instructions MCoN M822N-40
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Nervous System
Cell Apps Flyer Brain Cells
Cell Apps Poster Primary Cells
Brochure Cell Applications Inc

Description

MCoN (Mouse Cortical Neurons) are cryopreserved primary mouse neurons derived from the cerebral cortex of day 18 embryonic CD1 mouse brains. When cultured under the recommended conditions, MCoN arborize and form complex neurite network in one week. MCoN Stain positive for β III-Tubulin. 
 
Mouse cortical neurons are available in multiple formats, including cryopreserved neurons, freshly isolated primary neurons in suspension, or pre-plated cultured neurons.
 
The cerebral cortex plays a key role in cognition, memory, attention, perceptual awareness, thought, language and consciousness. Neurons connect to other neural cells through neurites and synapses to form complex neurocircuitry for signal transmission. A healthy circuitry in the brain is essential for cognitive functions, proper control and regulation of the other parts of the body. Dysfunction and degeneration in the cerebral cortex have been implicated in various brain injuries, neurological and psychiatric disorders. Cortical neurons, therefore, provide an excellent cellular model system to study disease mechanisms and pathophysiologies.  They also serve as a platform for drug screening and development, toxicity tests, immunostaining, live cell imaging, co-culturing and electrophysiology.
(Click to Enlarge) Freshly isolated Mouse Cortical Neurons (MCoN) cultured for 7 days. A: Phase contrast, 100x. B: Immunostaining for β-III Tubulin (green), GFAP (red), DAPI (blue), 100x.

Details

TissueNormal healthy mouse brain  
QCNo bacteria, yeast, fungi, mycoplasma
CharacterPositive for β-III Tubulin
BioassayPlate on Poly-D-Lysine coated surface, arborize to form neurite network in Culture Med
Cryovial2 or 4M MCoN cryopreserved after isolation in Serum-Free Freezing Medium (042-50)
Kit (2M)2M cryopreserved MCoN (M882N-20), Coating Soln I (027-05), Plating Med (M817P-20), Culture Med (M817-100)
Kit (4M)4M cryopreserved MCoN (M882N-40), Coating Soln I (027-10), Plating Med (M817P-40), Culture Med (M817-100)
Fresh CultureContact CAI if interested in freshly isolated MCoN (suspension or plated)
Plated2M MCoN from cryovial, in Culture Medium
DoublingsN/A - Neurons don't proliferate in vitro
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

Instructions MCoN M822N-20

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Instructions MCoN M822N-40

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved MCoN Total Kit: : 2x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: M882NK-20 Price: $543.00
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Cryopreserved MCoN Total Kit:: 4x10^6 Cells, Plating Medium, Culture Medium & Coating Solution (See Details tab for specifics) Size: 1 Kit CAT.#: M882NK-40 Price: $683.00
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Cryopreserved MCoN: : Frozen MCoN (2x10^6) Size: 1 Cryovial CAT.#: M882N-20 Price: $420.00
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Cryopreserved MCoN: : Frozen MCoN (4x10^6) Size: 1 Cryovial CAT.#: M882N-40 Price: $560.00
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Related Products

Product Size CAT.# Price Quantity
Mouse Neuron Culture Medium: All-in-one ready-to-use media Size: 100 ml CAT.#: M817-100 Price: $78.00
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Mouse Neuron Culture Medium Kit: Basal Medium and Growth Supplement sold together, bottled separatedly Size: Yields 100 ml CAT.#: M817K-100 Price: $86.00
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Mouse Neuron Plating Medium: For initial plating of neurons Size: 20 ml CAT.#: M817P-20 Price: $38.95
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Mouse Neuron Plating Medium: For initial plating of neurons Size: 10 ml CAT.#: M817P-10 Price: $20.45
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.14
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $30.75
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Neuron Transfection Kit: 100 x 24-Well Rxns Size: 1 Kit CAT.#: TF886K Price: $403.59
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Cytofect™ Neuron Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF886KS Price: $53.76
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Nervous System

Format: PDF

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Cell Apps Flyer Brain Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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