Instructions HSAEpC
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of lung tissue in the bronchiole area of the respiratory tract. Located in a region critical to gas exchange and immune response, HSAEpC provide an in vitro model to research cell biology, immunology and pharmaceutical drug discovery.  The small (pulmonary) epithelium forms a continuous lining in the airways, offering mechanical and immunological barriers against the external environment, physically protecting the underlying submucosa.
For general cell biology and airway function, the cells provide insights into pulmonary fluid balance, vascular and airway homeostasis and even the lung microbiome. Other work highlights epithelial cell junction integrity, and maintenance of the alveolar barrier integrity that provides first line host defense against inhaled pathogens. HSAEpC help unravel inflammatory signaling pathways, transcription factors and gene expression cascades. Decades of work in lung remodeling, wound healing and repair of surface airway epithelium damage have more recently paved the way to lung organoids and 3D models of small airway epithelium.
Primary HSAEpC offer physiological relevance to the study of respiratory infection, recognition, and immune responses to bacteria, viruses and other foreign invaders like nanoparticles, air pollution and cigarette smoke.  Such insults trigger production of chemokines, cytokines and lipids that mediate inflammation and recruit inflammatory cells. HSAEpC dysregulation has also be studied in terms of apoptosis, superoxide production and the effects of hypoxia or radiation.
HSAEpC are targeted in chronic diseases like bronchiolitis, asthma, COPD, cystic fibrosis, pulmonary hypertension and lung cancer.  Accordingly, the cells find increasing applications for drug discovery assays, in the hope to identify new therapeutic options to treat or prevent airway disorders.  Such tests included compound screening, toxicology, drug uptake and response, in formats ranging from standard 2D assays and microplates to 3D tissue models and airway-on-a-chip technology.
(Click to Enlarge) Human Small Airway Epithelial Cells (HSAEpC) play important roles in gas exchange, immune response and barrier function.  They are available cryopreserved, as fresh proliferating cells, or in convenient kits containing media and subculture reagents. Staining: CK18 (red) and CK19 (green).

Details

TissueNormal healthy human small airway
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HSAEpC (1st passage) frozen in Basal Med w/ 10% FBS, 10% DMSO
KitCryovial frozen HSAEpC (532-05a), Gr Med (512-500), Subculture Rgnt Kit (090K)
ProliferatingShipped in Gr Med, 3rd psg (flasks or plates)
DoublingsAt least 6
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HSAEpC

Format: PDF

Download Now

Products

Product Size CAT.# Price Quantity
Cryopreserved HSAEpC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 532K-05a Price: $853.00
-+
Cryopreserved HSAEpC, adult: Frozen HSAEpC (5x10^5) Size: 1 Cryovial CAT.#: 532-05a Price: $695.00
-+
Proliferating HSAEpC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 532-25a Price: $695.00
-+
Proliferating HSAEpC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 532-75a Price: $895.00
-+
Proliferating HSAEpC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 532-6Wa Price: $895.00
-+
Proliferating HSAEpC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 532-96Wa Price: $1,025.00
-+

Related Products

Product Size CAT.# Price Quantity
Human EpiVita Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 140-500 Price: $74.00
-+
Human Small Airway Epithelial Cell Growth Media: All-in-one ready-to-use Size: 500 ml CAT.#: 512-500 Price: $125.00
-+
Human Small Airway Epithelial Cell Growth Media Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 512K-500 Price: $135.00
-+
Human Small Airway Epithelial Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml CAT.#: 512-GS Price: $61.00
-+

Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
-+
Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
-+
Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $404.00
-+
Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $54.00
-+
Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $54.00
-+
Human Lung RNA: Total RNA prepared from human lung tissue Size: 250 ug CAT.#: 1H40-250 Price: $522.00
-+
Human Lung RNA: Total RNA prepared from human lung tissue Size: 50 ug CAT.#: 1H40-50 Price: $139.00
-+

Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

Downoad Now
5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now