Instructions HRPTEpC
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Renal Proximal Tubular Epithelial Cells (HRPTEpC) provide a useful tool for studying various aspects of pathology and biology of human kidney cells in vitro.  HRPTEpC from Cell Applications, Inc. have recently been used in a study of metabolic enzymes responding to energy depletion in renal cells, and they a pivotal role in kidney function including reabsorbtion of glucose and protein, and also play a part in the glucotoxicity associated with diabetes.
Characterization: Morphology consistent with epithelial origin, and positive for epithelial cell marker cytokeratin 18
(Click to Enlarge) Human Renal Proximal Tubular Epithelial Cells, HRPTEpC, Confocal image (L) and immunofluorescently stained with anti-CK19 (R)

Details

TissueNormal healthy human Kidney
QCNo bacteria, yeast, fungi, mycoplasma, virus
BioassayAttach, spread, proliferate in Growth Med
Cryovial500,000 HRPTEpC (1st or 2nd psg) frozen in Basal Med w/ 10% FBS, 10% DMSO
KitCryovial frozen HRPTEpC (930-05a), Growth Medium (911-500), Subcltr Rgnt Kit (090K)
ProliferatingShipped in Tsfr Med, psg 3, flasks or plates
DoublingsAt least 6
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HRPTEpC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HRPTEpC Total Kit: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 930K-05n Price: $844.72
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Cryopreserved HRPTEpC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 930K-05a Price: $834.23
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Cryopreserved HRPTEpC Total Kit: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 930K-05f Price: $844.72
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Cryopreserved HRPTEpC, adult: Frozen HRPTEpC (5x10^5) Size: 1 Cryovial CAT.#: 930-05a Price: $666.20
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Cryopreserved HRPTEpC, fetal: Frozen HRPTEpC (5x10^5) Size: 1 Cryovial CAT.#: 930-05f Price: $676.96
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Cryopreserved HRPTEpC, neonatal: Frozen HRPTEpC (5x10^5) Size: 1 Cryovial CAT.#: 930-05n Price: $676.96
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Proliferating HRPTEpC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 931-25a Price: $666.20
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Proliferating HRPTEpC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 931-75a Price: $870.69
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Proliferating HRPTEpC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 931-6Wa Price: $870.69
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Proliferating HRPTEpC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 931-96Wa Price: $999.84
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Proliferating HRPTEpC, fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 931-96Wf Price: $1,010.60
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Proliferating HRPTEpC, fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 931-25f Price: $676.96
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Proliferating HRPTEpC, fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 931-75f Price: $881.45
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Proliferating HRPTEpC, fetal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 931-6Wf Price: $881.45
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Proliferating HRPTEpC, neonatal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 931-25n Price: $676.96
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Proliferating HRPTEpC, neonatal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 931-75n Price: $881.45
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Proliferating HRPTEpC, neonatal: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 931-6Wn Price: $881.45
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Proliferating HRPTEpC, neonatal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 931-96Wn Price: $1,010.60
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Related Products

Product Size CAT.# Price Quantity
Human RenaEpi Basal Medium : Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 910-500 Price: $64.58
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Human RenaEpi Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 911-500 Price: $123.77
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Human RenaEpi Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 911K-500 Price: $134.53
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Human RenaEpi Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml (3 parts) CAT.#: 911-GS Price: $69.96
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-IL-1Ra: Monoclonal IL-1 Receptor Antagonist Antibody Size: 100 ul CAT.#: CB19846 Price: $302.38
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Epithelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF102KS Price: $53.76
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Cytofect™ Epithelial Cell Transfection Kit: 124 x 24-Well Rxns Size: 1 Kit CAT.#: TF102K Price: $403.59
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HRPTEpC RNA: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, adult Size: 10 ug CAT.#: 930-R10a Price: $452.03
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HRPTEpC RNA: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, adult Size: 25 ug CAT.#: 930-R25a Price: $904.05
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HRPTEpC RNA: Human Renal Proximal Tubular Epithelial Cells RNA, fetal Size: 10 ug CAT.#: 930-R10f Price: $408.98
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HRPTEpC RNA: Total RNA prepared from Human Renal Proximal Tubular Epithelial Cells, fetal Size: 25 ug CAT.#: 930-R25f Price: $817.95
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 50 ug CAT.#: 1H50-50 Price: $138.57
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Human Kidney RNA: Total RNA prepared from human kidney tissue Size: 250 ug CAT.#: 1H50-250 Price: $521.98
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IL-1α / IL-1F1: Human Interleukin-1-alpha Size: 10 ug CAT.#: RP1173-10 Price: $193.73
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IL-1α / IL-1F1: Human Interleukin-1-alpha Size: 100 ug CAT.#: RP1173-100 Price: $624.23
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IL-1α / IL-1F1: Human Interleukin-1-alpha Size: 1000 ug CAT.#: RP1173-1000 Price: $5,327.44
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IL-1α ELISA Kit, Human: Human Interleukin-1 alpha ELISA Kit Size: 96 wells CAT.#: CL0389 Price: $484.31
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IL-1β ELISA Kit, Human: Human Interleukin-1 beta ELISA Kit Size: 96 wells CAT.#: CL0392 Price: $586.56
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017
Bidwell, G., F. Mahdi, Q. Shao, O. Logue, J. Waller, C. Reese and A. Chade. 2017. A kidney-selective biopolymer for targeted drug deliveryAm J Physiol Renal Physiol, 312: F54-F64.
2016
Ito, H., O. Ichiyanagi, S. Naito, V. Bilim, Y. Tomita, Y. Kato, A. Nagaoka and N. Tsuchiya. 2016. GSK-3 directly regulates phospho-4EBP1 in renal cell carcinoma cell-line: an intrinsic subcellular mechanism for resistance to mTORC1 inhibition. BMC Cancer 16:393.
2014
Jackson, E., D. Gillespie, Z. Mi, D. Cheng, R. Bansal, K. Janesko-Feldman and P. Kochanek. 2014.Role of 2′,3′-cyclic nucleotide 3′-phosphodiesterase in the renal 2′,3′-cAMP-adenosine pathway. Am J Physiol – Renal Physiol, 307:F14-F24.
2012

 

Jackson, E. and D. Gillespie. 2012. Extracellular 2′,3′-cAMP and 3′,5′-cAMP stimulate proliferation of preglomerular vascular endothelial cells and renal epithelial cells. Am J Physiol – Renal Physiol, 303:F954-F962.