Instructions PBMC (HMNC-PB)
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
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Description
Peripheral Blood Mononuclear Cells (PBMC), also known as Human Mononuclear Cells from Peripheral Blood (HMNC-PB), are widely used in research and clinical applications every day, and provide a useful tool for studying various aspects of pathology and biology in vitro. Cell Applications PBMC are cryopreserved immediately after isolation.
PBMC / HMNC-PB are important tools for studies in:
- Immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology
- High-throughput screening
- Neovascularization to improve limb ischemia and in cell therapy to repair ischemic and infracted myocardium.
- Human body defense mechanisms critical in the immune system to fight bacterial and viral infections, and function as immune surveillance network to kill tumor cells.
- The secretion of cytokines to regulate immune response and inflammation
- Biological effects on tissue and organs.
- Chemokine effects on adipocytes and muscles
(Click to Enlarge) Peripheral Blood Mononuclear Cells: PBMC, also known as Human Mononuclear Cells from Peripheral Blood (HMNC-PB).
Details
Tissue | Normal healthy human peripheral blood |
QC | No bacteria, yeast, fungi, mycoplasma, virus |
Cryovial | 10,000,000 PBMC / HMNC-PB frozen right after isolation in DMEM w/ 45% FBS, 10% DMSO |
Kit | Cryovial Frozen PBMC / HMNC-PB, Culture Medium (615-250) |
| Doublings | N/A - Blood cells don't proliferate in vitro |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Mononuclear Cells-Periphery Blood Total Kit, adult: 1x10^7 Cells (Adult) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: 690PBK-100a | Price: $344.00 | |
| Cryopreserved Peripheral Blood Cells (PBMC/HMNC-PB), adult: Frozen PBMC (1x10^7) | Size: 1 Cryovial | CAT.#: 690PB-100a | Price: $278.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Blood Cell Culture Medium: Optimized and ready for use. | Size: 250 ml | CAT.#: 615-250 | Price: $76.00 | |
| Human Blood Cell Culture Medium Kit: Basal medium & supplement sold together packaged separately | Size: Yields 250 ml | CAT.#: 615K-250 | Price: $87.00 | |
| Human Blood Cell Basal Medium: Basal medium (contains no growth supplement). Add Supplement before use. | Size: 250 ml | CAT.#: 614-250 | Price: $49.00 | |
| Human Blood Cell Culture Supplement: Added to Basal Medium to create Culture (Maintenance) Medium | Size: 40 ml | CAT.#: 615-CS | Price: $52.00 | |
| Hematopoietic Cell Freezing Medium: Serum-free freezing medium optimized for Peripheral Blood Mononuclear Cells (PBMC) | Size: 50 ml | CAT.#: 041-50 | Price: $85.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Mouse Cluster of Differentiation 3 Antibody: Mouse Cluster of Differentiation 3 Antibody | Size: 100 ul | CAT.#: CP10034 | Price: $302.00 | |
| Mouse Cluster of Differentiation 4 Antibody: Mouse Cluster of Differentiation 4 Antibody | Size: 100 ul | CAT.#: CP10035 | Price: $302.00 | |
| Mouse CD8 monoclonal Antibody: Mouse CD8 monoclonal antibody | Size: 100 ul | CAT.#: CP10036 | Price: $302.00 | |
| Anti-CD8: Rabbit CD8 antibody | Size: 100 ul | CAT.#: CG1649 | Price: $289.00 | |
| Freezing Medium for Blood Cells: Freezing Medium optimized for the unique needs of Blood Cells | Size: 50 ml | CAT.#: 040-50BC | Price: $75.00 | |
| Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 10 ug | CAT.#: RP1021-10 | Price: $194.00 | |
| Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 100 ug | CAT.#: RP1021-100 | Price: $484.00 | |
| Human FMS-Related Tyrosine Kinase 3 Ligand (FLT-3 ligand): Human FMS-related Tyrosine Kinase 3 Ligand | Size: 1000 ug | CAT.#: RP1021-1000 | Price: $4,090.00 | |
| Cryopreserved Human Peripheral Blood CD19+ B Cells Total Kit, adult: 2x10^6 CD19+ B Cells (Adult) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: 6904K-20a | Price: $991.00 | |
| Cryopreserved Peripheral Blood B Cells (HPBB), adult: Frozen HPBB, CD19+ (2x10^6) | Size: 1 Cryovial | CAT.#: 6904-20a | Price: $925.00 | |
| Cryopreserved Human Peripheral Blood CD4+ T Cells Total Kit, adult: 5x10^6 Cells CD4+ T Cells (Adult) & Medium (See Details tab for specifics) | Size: 1 Kit | CAT.#: 6902K-50a | Price: $891.00 | |
| Cryopreserved Peripheral Blood T Cells (HPBT), adult: Frozen HPBT, CD4+ | Size: 1 Cryovial | CAT.#: 6902-50a | Price: $825.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 | |
| Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 10 ug | CAT.#: RP1021AF-10 | Price: $213.00 | |
| Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 100 ug | CAT.#: RP1021AF-100 | Price: $533.00 | |
| Human FLT-3 ligand, Animal-Free: Human FMS-Related Tyrosine Kinase 3 Ligand, Animal-Free | Size: 1000 ug | CAT.#: RP1021AF-1000 | Price: $4,499.00 |
Resources/Documents
Publications
2017
Ota, K. and S. Tanaka. 2017. ANIMAL CELL CULTURE MEDIUM AND CULTURE CONTAINER. Patent Application 20170022477 A1.