MSDS Cryopreserved Cells
Instructions HOb-OA
Cell Apps Flyer Skeletal System Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells

Description

Human Osteoblasts-Osteoarthritis (HOb-OA) are isolated from bone of patients with osteoarthritis (OA) and provide an excellent model system to study the pathophisiology of that disease in vitro, including matrix mineralization, effects of inflammatory mediators and bone morphogenic proteins, physiological control of bone remodeling and regulation of bone metabolism.
 
Characterization: Positive for bone mineralization.
(Click to Enlarge) Human Osteoblasts-Osteoarthritis: HOb-OA. Bone forming cells, isolated from donor patient with OA.

Details

Tissue
Bone from donors w/ osteoarthritis
QC
No bacteria, yeast, fungi, mycoplasma, virus 
Cryovial
500,000 HOb-OA (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HOb-OA (406OA-05), Growth Medium (417-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HOb-OA

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HOb-OA, Adult: 5x10^5 Cells (from donor with Osteoarthitis, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 406OAK-05a Price: $909.00
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Cryopreserved HOb-OA, Adult: Frozen HOb-OA (5x10^5) Size: 1 Cryovial CAT.#: 406OA-05a Price: $748.00
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Proliferating HOb-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: T-25 Flask CAT.#: 407OA-25a Price: $748.00
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Proliferating HOb-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: T-75 Flask CAT.#: 407OA-75a Price: $952.00
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Proliferating HOb-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: 24 Well CAT.#: 407OA-24Wa Price: $914.00
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Proliferating HOb-OA, Adult: Actively growing, dividing cells in medium, from donor with Osteoarthritis Size: 96 Well CAT.#: 407OA-96Wa Price: $1,043.00
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Related Products

Product Size CAT.# Price Quantity
HOb Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 416-500 Price: $62.00
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HOb Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 417D-250 Price: $120.00
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HOb Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 417-500 Price: $116.00
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HOb Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 417K-500 Price: $125.00
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HOb Growth Medium, wo FBS: Growth medium without FBS Size: 500 ml CAT.#: 417F-500 Price: $125.00
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HOb Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 417-GS Price: $62.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Extended Family Products

Product Size CAT.# Price Quantity
Monoclonal Bone Morphogenic Protein 4, BMP-4, Antibody: Monoclonal Bone Morphogenic Protein 4, BMP-4, Antibody Size: 100 ul CAT.#: CB12470 Price: $302.00
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Mouse BMPR2 Antibody: Mouse BMPR2 Antibody Size: 100 ul CAT.#: CP10368 Price: $302.00
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Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 Size: 10 ug CAT.#: RP1053-10 Price: $194.00
Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 Size: 100 ug CAT.#: RP1053-100 Price: $624.00
Human Bone Morphogenetic Protein-2 (BMP-2): Human Bone Morphogenetic Protein-2 Size: 1000 ug CAT.#: RP1053-1000 Price: $5,327.00
Human BMP-2 ELISA Kit: Human Bone Morphogenic Protein-2 ELISA Kit Size: 96 Wells CAT.#: CL0311 Price: $500.00
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Human BMP-4 ELISA Kit: Human Bone Morphogenic Protein-4 ELISA Kit Size: 96 Wells CAT.#: CL0314 Price: $457.00
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Human BMP-5 ELISA Kit: Human Bone Morphogenic Protein-5 ELISA Kit Size: 96 Wells CAT.#: CL0310 Price: $500.00
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Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor Size: 20 ug CAT.#: RP1060-20 Price: $194.00
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Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor Size: 100 ug CAT.#: RP1060-100 Price: $484.00
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Human Connective Tissue Growth Factor (CTGF): Human Connective Tissue Growth Factor Size: 1000 ug CAT.#: RP1060-1000 Price: $4,090.00
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HOb RNA, Adult: Total RNA prepared from Human Osteoblasts, adult Size: 10 ug CAT.#: 406-R10a Price: $452.00
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HOb RNA, Adult: Total RNA prepared from Human Osteoblasts, adult Size: 25 ug CAT.#: 406-R25a Price: $904.00
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HOb RNA, Fetal: Total RNA prepared from Human Osteoblasts, fetal Size: 25 ug CAT.#: 406-R25f Price: $700.00
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HOb Differentiated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Differentiated, fetal Size: 10 ug CAT.#: 406D-R10f Price: $409.00
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HOb Differentiated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Differentiated, fetal Size: 25 ug CAT.#: 406D-R25f Price: $818.00
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HOb Stimulated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Stimulated, fetal Size: 10 ug CAT.#: 406S-R10f Price: $452.00
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HOb Stimulated RNA, Fetal: Total RNA prepared from Human Osteoblasts, Stimulated, fetal Size: 25 ug CAT.#: 406S-R25f Price: $904.00
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Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand Size: 10 ug CAT.#: RP1019-10 Price: $194.00
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Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand Size: 100 ug CAT.#: RP1019-100 Price: $624.00
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Human Soluble Receptor Activator of NF-kB Ligand (RANK-Ligand): Human soluble Receptor Activator of NFκB Ligand Size: 1000 ug CAT.#: RP1019-1000 Price: $5,327.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free Size: 10 ug CAT.#: RP1019AF-10 Price: $213.00
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Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free Size: 100 ug CAT.#: RP1019AF-100 Price: $687.00
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Human RANK-Ligand, Animal-Free: Human Soluble Receptor Activator of NFκB Ligand, Animal-Free Size: 1000 ug CAT.#: RP1019AF-1000 Price: $5,860.00
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Resources/Documents

Cell Apps Flyer Skeletal System Cells

Format: PDF

Downoad Now
5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Publications

2006
Ando, T., J. Ichikawa, M. Wako, K. Hatsushika, Y. Watanabe, M. Sakuma, K. Tasaka, H. Ogawa, Y. Hamada, H. Yagita, and A. Nakao. 2006. TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells. Arthritis research & therapy. 8:R146.