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Human Internal Thoracic Artery Endothelial Cells: HITAEC

(Click to Enlarge) Human Internal Thoracic Endothelial Cells: HITAEC (L) immunolabeled for vWF (green) and CD31/PECAM (red) (M). Nuclei visualized with PI (red) (R).

MSDS Cryopreserved Cells
Instructions HITAEC
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells
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 Human Internal Thoracic Artery Endothelial Cells (HITAEC) from Cell Applications, Inc. have been used to

  • Investigate how oral pathogens travel through the body by infecting dendritic cells and inducing them to migrate through endothelial layers by causing upregulation of CXCR4
  • Identify genes that make Human Internal Thoracic Arteries resistant to atherosclerosis
Additionally, HITAEC and HCAEC (also obtained from Cell Applications, Inc. but derived from the athero-prone human coronary arteries) were subjected to microarray analysis.  Twenty-nine genes were found to be expressed differently between HITAEC and HCAEC; one of them (TES) was shown to be downregulated in patients with coronary artery disease, resulting in increased monocyte adhesion, endothelial cell motility and transendothelial migration of monocytes.
Tissue
Normal healthy human mammary artery
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ag & DiI-Ac-LDL uptake
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HITAEC (2nd psg) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HITAEC (308-05a), Growth Medium (212-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
2014
Miles, B., I. Zakhary, A. El-Awady, E. Scisci, J. Carrion, J.C. O'Neill, A. Rawlings, J.K. Stern, C. Susin, and C.W. Cutler. 2014. Secondary Lymphoid Organ Homing Phenotype of Human Myeloid Dendritic Cells Disrupted by an Intracellular Oral Pathogen. Infect. Immun. 82:101-111.
2012
Archacki, S.R., G. Angheloiu, C.S. Moravec, H. Liu, E.J. Topol, and Q.K. Wang. 2012. Comparative gene expression analysis between coronary arteries and internal mammary arteries identifies a role for the TES gene in endothelial cell functions relevant to coronary artery disease. Human molecular genetics. 21:1364-1373.
Colter, D., C. Buensuceso, C. Kazanecki, and A. Gosiewska. 2012. Mammary artery derived cells and methods of use in tissue repair and regeneration. Patent US 8323972 B2.

Products

Cryopreserved HITAEC Total Kit

Pricing
Description
Size
Cat. #
Price
Cryopreserved HITAEC Total Kit 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) 1 Kit 308K-05a $898.24

Cryopreserved HITAEC, adult

Pricing
Description
Size
Cat. #
Price
Cryopreserved HITAEC, adult Frozen HITAEC (5x10^5) 1 Cryovial 308-05a $747.99

Proliferating HITAEC, adult

Pricing
Description
Size
Cat. #
Price
Proliferating HITAEC, adult Actively growing, dividing cells in medium T-25 Flask 309-25a $747.99
Proliferating HITAEC, adult Actively growing, dividing cells in medium T-75 Flask 309-75a $952.48
Proliferating HITAEC, adult Actively growing, dividing cells in medium 6 Well 309-6Wa $952.48
Proliferating HITAEC, adult Actively growing, dividing cells in medium 96 Well 309-96Wa $1,081.63

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