MSDS Cryopreserved Cells
Instructions HITAEC
5 Important Cell Culture Rules
Cell Apps Flyer Cardiovascular Cells
Cell Apps Flyer Endothelial Cells
Cell Apps Poster Primary Cells

Description

 Human Internal Thoracic Artery Endothelial Cells (HITAEC) from Cell Applications, Inc. have been used to

  • Investigate how oral pathogens travel through the body by infecting dendritic cells and inducing them to migrate through endothelial layers by causing upregulation of CXCR4
  • Identify genes that make Human Internal Thoracic Arteries resistant to atherosclerosis
Additionally, HITAEC and HCAEC (also obtained from Cell Applications, Inc. but derived from the athero-prone human coronary arteries) were subjected to microarray analysis.  Twenty-nine genes were found to be expressed differently between HITAEC and HCAEC; one of them (TES) was shown to be downregulated in patients with coronary artery disease, resulting in increased monocyte adhesion, endothelial cell motility and transendothelial migration of monocytes.

(Click to Enlarge) Human Internal Thoracic Endothelial Cells: HITAEC (L) immunolabeled for vWF (green) and CD31/PECAM (red) (M). Nuclei visualized with PI (red) (R).

Details

Tissue
Normal healthy human mammary artery
QC
No bacteria, yeast, fungi, mycoplasma, virus
Character
Factor VIII-related Ag & DiI-Ac-LDL uptake
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HITAEC (2nd psg) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HITAEC (308-05a), Growth Medium (212-500), Subculture Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 3rd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HITAEC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HITAEC Total Kit: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 308K-05a Price: $898.24
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Cryopreserved HITAEC, adult: Frozen HITAEC (5x10^5) Size: 1 Cryovial CAT.#: 308-05a Price: $747.99
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Proliferating HITAEC, adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 309-25a Price: $747.99
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Proliferating HITAEC, adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 309-75a Price: $952.48
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Proliferating HITAEC, adult: Actively growing, dividing cells in medium Size: 6 Well CAT.#: 309-6Wa Price: $952.48
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Proliferating HITAEC, adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 309-96Wa Price: $1,081.63
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Related Products

Product Size CAT.# Price Quantity
Human MesoEndo Cell Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 212-500 Price: $118.39
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Human MesoEndo Cell Growth Medium: Growth medium without phenol red Size: 500 ml CAT.#: 212PR-500 Price: $118.39
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Human MesoEndo Cell Growth Medium: Growth medium without antibiotics Size: 500 ml CAT.#: 212A-500 Price: $123.77
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Human MesoEndo Cell Growth Medium: Growth Medium without FBS Size: 500 ml CAT.#: 212F-500 Price: $136.33
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Human MesoEndo Cell Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500ml CAT.#: 212K-500 Price: $118.39
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Human MesoEndo Cell Growth Medium Kit: Growth medium kit without phenol red Size: yields 500 ml CAT.#: 212KPR-500 Price: $130.23
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Human MesoEndo Cell Growth Supplement: Added to Basal Medium to create Growth Medium Size: 30 ml CAT.#: 212-GS Price: $62.42
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Human MesoEndo Cell Growth Supplement: Growth Supplement without FBS Size: 5 ml CAT.#: 212F-GS Price: $68.00
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-ICAM-1: Rabbit Intercellular Adhesion Molecule-1 Antibody Size: 100 ul CAT.#: CG1238 Price: $275.00
Anti-VEGF: Polyclonal Vascular Endothelial Growth Factor Antibody Size: 100 ul CAT.#: CA1080 Price: $302.38
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Anti-VEGF-C: Polyclonal Vascular Endothelial Growth Factor-C Antibody Size: 100 ul CAT.#: CB3778 Price: $302.38
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Anti-VEGFR1: Polyclonal VEGF Receptor 1 Antibody Size: 100 ul CAT.#: CB3839 Price: $333.13
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Endothelial Cell Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF101KS Price: $53.76
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Cytofect™ Endothelial Cell Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF101K Price: $430.50
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E-Selectin ELISA Kit, Human: Human E-Selectin ELISA Kit Size: 96 Wells CAT.#: CL0501 Price: $586.56
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HITAEC RNA: Total RNA prepared from Human Internal Thoracic Artery Endothelial Cells, adult Size: 10 ug CAT.#: 308-R10a Price: $408.98
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HITAEC RNA: Total RNA prepared from Human Internal Thoracic Artery Endothelial Cells, adult Size: 25 ug CAT.#: 308-R25a Price: $817.95
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $138.57
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $521.98
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ICAM-1 ELISA Kit, Human: Human Intercellular Adhesion Molecule-1 ELISA Kit Size: 96 Wells CAT.#: CL0370 Price: $484.31
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,174.94
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $193.73
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IP-10 / CXCL10: Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.31
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P-Selectin ELISA Kit, Human: Human P-Selectin ELISA Kit Size: 96 wells CAT.#: CL0505 Price: $516.60
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 10 ug CAT.#: RP1116-10 Price: $193.73
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VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 100 ug CAT.#: RP1116-100 Price: $484.31
-+
VEGF-121: Human Vascular Endothelial Growth Factor-121 Size: 1000 ug CAT.#: RP1116-1000 Price: $4,089.75
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VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 10 ug CAT.#: RP1116AF-10 Price: $213.10
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VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 100 ug CAT.#: RP1116AF-100 Price: $532.74
-+
VEGF-121, Animal-Free: Human Vascular Endothelial Growth Factor-121, Animal-Free Size: 1000 ug CAT.#: RP1116AF-1000 Price: $4,498.73
-+
VEGF-C ELISA Kit, Human: Human Vascular Endothelial Growth Factor C ELISA Kit Size: 96 Wells CAT.#: CL0588 Price: $581.18
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Cardiovascular Cells

Format: PDF

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Cell Apps Flyer Endothelial Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Publications

2014
Miles, B., I. Zakhary, A. El-Awady, E. Scisci, J. Carrion, J.C. O'Neill, A. Rawlings, J.K. Stern, C. Susin, and C.W. Cutler. 2014. Secondary Lymphoid Organ Homing Phenotype of Human Myeloid Dendritic Cells Disrupted by an Intracellular Oral Pathogen. Infect. Immun. 82:101-111.
2012
Archacki, S.R., G. Angheloiu, C.S. Moravec, H. Liu, E.J. Topol, and Q.K. Wang. 2012. Comparative gene expression analysis between coronary arteries and internal mammary arteries identifies a role for the TES gene in endothelial cell functions relevant to coronary artery disease. Human molecular genetics. 21:1364-1373.
Colter, D., C. Buensuceso, C. Kazanecki, and A. Gosiewska. 2012. Mammary artery derived cells and methods of use in tissue repair and regeneration. Patent US 8323972 B2.