MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure
Instructions HHStellateCells
Description
Hepatic Stellate Cells (HHSC) are one of the major cell type that reside between hepatocytes and blood vessels in the liver. HHSCs play an important role in liver homeostasis, maintenance and regeneration including retinol metabolism. Activation of stellate cells in disease state leads to liver fibrosis, hepatitis B & C, fatty liver disease, diabetes and other chronic diseases. Hepatic Stellate Cells are primary cells derived from human liver and cultured in optimized Hepatic Stellate Cells Growth Medium. Primary culture of Hepatic Stellate Cells is suitable for many cell-based assays including toxicity, drug screening and metabolism and is a valuable tool to study liver function, physiology and liver diseases
Characterization: Positive for α-smooth muscle actin and fibronectin by ICC staining.
Human Hepatic Stellate Cells: HHSC Left: HHSC immunolabeled for α-smooth muscle actin (green) Right: HHSC immunostained for fibronectin (green) nuclei are stained with DAPI.
Details
| Tissue | Normal healthy human liver tissue |
| QC | No bacteria, yeast, fungi, mycoplasma, virus |
| Bioassay | Attach, spread, proliferate in Growth Med |
| Cryovial | 500,000 HHSC (2nd passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO |
| Kit | Cryovial frozen HHSC (782-05f), Growth Medium (7117-500), Subcltr Rgnt Kit (090K) |
| Proliferating | Shipped in Gr Med, 2nd psg (flasks or plates) |
| Doublings | At least 15 |
| Applications | Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use. |
Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Cryopreserved Human Hepatic Stellate Cells Total Kit, fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) | Size: 1 Kit | CAT.#: 782K-05f | Price: $903.00 | |
| Cryopreserved Hepatic Stellate Cells (HHSC), fetal: Frozen HHSC (5x10^5) | Size: 1 Cryovial | CAT.#: 782-05f | Price: $725.00 | |
| Proliferating Hepatic Stellate Cells (HHSC), fetal: Actively growing, dividing cells in medium | Size: T-25 Flask | CAT.#: 783-25f | Price: $725.00 | |
| Proliferating Hepatic Stellate Cells (HHSC), fetal: Actively growing, dividing cells in medium | Size: T-75 Flask | CAT.#: 783-75f | Price: $915.00 | |
| Proliferating Hepatic Stellate Cells (HHSC), fetal: Actively growing, dividing cells in medium | Size: 96 Well | CAT.#: 783-96Wf | Price: $1,035.00 |
Related Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Human Hepatic Stellate Cells Growth Medium: All-in-one ready-to-use | Size: 500 ml | CAT.#: 7117-500 | Price: $125.00 | |
| Human Hepatic Stellate Cells Growth Medium Kit: Basal medium & growth supplement sold together packaged separately | Size: Yields 500 ml | CAT.#: 7117K-500 | Price: $135.00 | |
| Human Hepatic Stellate Cells Growth Supplement: Added to Basal Medium to create Growth Medium | Size: 50 ml | CAT.#: 7117-GS | Price: $68.00 | |
| Human Hepatic Stellate Cells Basal Medium: Basal medium (contains no growth supplement). Add GS before use. | Size: 500 ml | CAT.#: 7116-500 | Price: $74.00 |
Extended Family Products
| Product | Size | CAT.# | Price | Quantity |
|---|---|---|---|---|
| Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution | Size: 1 Kit | CAT.#: 090K | Price: $63.00 | |
| Cyto-X Cell Counting Reagent: 500 tests | Size: 1 Bottle | CAT.#: 028-01 | Price: $160.00 | |
| Cyto-X Cell Counting Reagent Sample: 100 tests | Size: Sample | CAT.#: 028-S | Price: $41.00 |