Instructions Gastrointestinal EpC
MSDS Cryopreserved Cells
Cell Apps Flyer Epithelial Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Now available: Adult Human Gastric Epithelial Cells

Adult HGaEpC Monolayer. Top left: Phase contrast; Top right(red): ZO-1; Bottom left (green): Na+K+ ATPase; Bottom middle (red): pan-Cytokeratin; Bottom right (green): Villin; Blue: DAPI

 
 
 

The gastric mucosa is the mucous membrane layer of the stomach lumenwhich contains the glands and the gastric pits. The human stomach lumen is lined with a monolayer of epithelial cells. The epithelium constantly renews itself and the stem cells fueling this process reside in the gastric glands. An important function of the gastrointestinal epithelium is to protect from infections, and support a peaceful co-existence with gut microbiota. The gastric pathogen Helicobacter pylori is one of the most common pathogens to colonize the stomach, and chronic infection can lead to gastric ulcers, gastritis, and gastric cancer. Primary human gastric epithelial cells would represent the gastric epithelium closely, but until now no primary gastric culture system exists that enables effective research of gastric diseases and disorders in vitro.

CAI provides a gastric epithelial culture system that results in a gastric epithelial cell monolayer with tight junction that is stable for up to a week in culture. Our system enables investigation of gastric epithelial cell physiology and their response to infection, tight junction function, as well as testing of multiple therapeutic agents for gastrointestinal research.

With optimized, defined culture media from CAI, the Gastric Epithelial Cells can be seeded and maintained for as long as 8 days. Epithelial Cells grown in CAI medium form a monolayer of polarized epithelial cells with tight junction formation as evidenced by Villin (apical marker), Na+/K+ ATPase (basolateral marker), ZO-1 (tight junction marker) and pan-Cytokeratin (epithelial marker) staining.

 

Human Gastric Epithelial Cell (HGaEpC) monolayer. Top left: Phase contrast. Top right (Red): ZO-1. Bottom left (Green): Villin. Bottom middle (Red): Na+/K+ ATPase. Bottom right (Green)  pan-Cytokeratin. Blue: DAPI.

Details

TissueNormal healthy human stomach.
QCNo bacteria, yeast, fungi, mycoplasma, virus.
BioassayAttach, spread, in Culture Medium.
Cryovial1,000,000 or 2,000,000 HGaEpC in Freezing Medium.
KitCryovial frozen HGaEpC (732Ga-20f), Culture Med (716DCM-50), Coating Solution (025-05), Thawing Solution (716T-20).
CulturedShipped in flasks or plates in medium.
DoublingsCells do not divide and cannot be passaged.
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions Gastrointestinal EpC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HGaEpC Total Kit, adult: 1x10^6 Cells (adult), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732GaK-10a Price: $852.00
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Cryopreserved HGaEpC Total Kit, adult: 5x10^5 Cells (adult), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732GaK-05a Price: $685.00
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Cryopreserved HGaEpC Total Kit, fetal: 1x10^6 Cells (fetal), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732GaK-10f Price: $752.00
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Cryopreserved HGaEpC Total Kit, fetal: 5x10^5 Cells (fetal), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732GaK-05f Price: $635.00
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Cryopreserved HGaEpC, adult: Frozen HCnEpC (5x10^5) Size: 1 Cryovial CAT.#: 732Ga-05a Price: $595.00
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Cryopreserved HGaEpC, adult: Frozen HGaEpC (1x10^6) Size: 1 Cryovial CAT.#: 732Ga-10a Price: $762.00
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Cryopreserved HGaEpC, fetal: Frozen HGaEpC (1x10^6) Size: 1 Cryovial CAT.#: 732Ga-10f Price: $662.00
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Cryopreserved HGaEpC, fetal: Frozen HGaEpC (5x10^5) Size: 1 Cryovial CAT.#: 732Ga-05f Price: $545.00
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Cultured HGaEpC, adult: Pre-plated, cultured cells in medium Size: - CAT.#: 733Ga-XXa Price: $0.00
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Cultured HGaEpC, fetal: Pre-plated, cultured cells in medium Size: - CAT.#: 733Ga-XXf Price: $0.00
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Related Products

Product Size CAT.# Price Quantity
GI Epithelial Cell Coating Solution: Coating solution for GI Epithelial Cells Size: 5 ml CAT.#: 025-05 Price: $16.00
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GI Epithelial Cell Defined Culture Medium: All-in-one ready-to-use. Use within 30 days of receipt. Size: 50 ml CAT.#: 716DCM-50 Price: $55.00
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GI Epithelial Cell Defined Culture Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 50 ml CAT.#: 716DCMK-50 Price: $75.00
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GI Epithelial Cell Thawing Solution: Thawing solution for GI Epithelial Cells Size: 20 ml CAT.#: 716T-20 Price: $26.00
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TEER Assay GI Epithelial Cell Medium : All-in-one ready-to-use optimized media for studying GI epithelial barrier function   Size: 50 ml CAT.#: 716TaM-50 Price: $65.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now