Instructions Gastrointestinal EpC
Cell Apps Flyer Epithelial Cells
MSDS Cryopreserved Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

The organization of the epithelium of the small intestine and colon into crypts is generally comparable. However, histologically there are two important differences between the two types of epithelia.The colonic epithelium has a flat epithelium surface and does not have villi. Regeneration occurs from units embedded within the crypts, each containing about 6–10 stem cells, while differentiated epithelial cells reside at the surface.

Human colonic epithelium is linked to various human diseases and disorders such as Ulcerative Colitis and Colon Cancer, necessitating a better understanding of epithelial cell function and their role in disease-affected tissues. CAI’s colonic epithelial culture system provides a unique tool for the investigation of colon epithelial cell physiology and evaluation of therapeutic mediators for translational research.

With optimized, defined culture media from CAI, the Colonic Epithelial Cells can be seeded and maintained for as long as 8 days. Epithelial Cells grown in CAI medium form a monolayer of polarized epithelial cells with tight junction formation as evidenced by Villin (apical marker), Na+/K+ ATPase (basolateral marker), ZO-1 (tight junction marker) and pan-Cytokeratin (epithelial marker) staining.

Human Colonic Epithelial Cell (HCnEpC) monolayer. Top left: Phase contrast. Top right (Red): ZO-1. Bottom left (Green): Villin. Bottom middle (Red): Na+/K+ ATPase. Bottom right (Green)  pan-Cytokeratin. Blue: DAPI.

Details

TissueNormal healthy human colon.
QCNo bacteria, yeast, fungi, mycoplasma, virus.
BioassayAttach, spread, in Culture Medium.
Cryovial5000,000, 1,000,000 HCnEpC in Freezing Medium.
KitCryovial frozen HCnEpC (732Cn-20f), Culture Med (716DC-50), Coating Solution (025-05), Thawing Solution (716T-20).
CulturedShipped in flasks or plates in medium.
DoublingsCells do not divide, and cannot be passaged.  Cells can be cultured up to 7 days.
ApplicationsLaboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions Gastrointestinal EpC

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Products

Product Size CAT.# Price Quantity
Cryopreserved HCnEpC, fetal: 1x10^6 Cells (fetal), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732CnK-10f Price: $912.00
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Cryopreserved HCnEpC, fetal: Frozen HCnEpC (1x10^6) Size: 1 Cryovial CAT.#: 732Cn-10f Price: $825.00
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Cryopreserved HCnEpC, fetal: 5x10^5 Cells (fetal), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732CnK-05f Price: $632.00
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Cryopreserved HCnEpC, fetal: Frozen HCnEpC (5x10^5) Size: 1 Cryovial CAT.#: 732Cn-05f Price: $545.00
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Cryopreserved HCnEpC, adult : Frozen HCnEpC (5x10^5) Size: 1 Cryovial CAT.#: 732Cn-05a Price: $595.00
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Cryopreserved HCnEpC, adult: 1x10^6 Cells (adult), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732CnK-10a Price: $982.00
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Cryopreserved HCnEpC, adult : Frozen HCnEpC (1x10^6) Size: 1 Cryovial CAT.#: 732Cn-10a Price: $895.00
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Cryopreserved HCnEpC, adult: 5x10^5 Cells (adult), Culture Medium, Coating and Thawing Solution (See Details tab for specifics) Size: 1 Kit CAT.#: 732CnK-05a Price: $682.00
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Cultured HCnEpC, fetal: Pre-plated, cultured cells in medium. Contact us at techsupport@cellapplications.com to order. Size: - CAT.#: 733Cn-XXf Price: $0.00
Cultured HCnEpC, adult : Pre-plated, cultured cells in medium. Contact us at techsupport@cellapplications.com to order. Size: - CAT.#: 733Cn-XXa Price: $0.00

Related Products

Product Size CAT.# Price Quantity
GI Epithelial Cell Defined Culture Medium: All-in-one medium, includes antibiotics. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716DC-50 Price: $55.00
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GI Epithelial Cell Coating Solution: Coating solution for GI Epithelial Cells Size: 5 ml CAT.#: 025-05 Price: $16.00
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GI Epithelial Cell Thawing Solution: Thawing solution for GI Epithelial Cells Size: 20 ml CAT.#: 716T-20 Price: $26.00
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Extended Family Products

Product Size CAT.# Price Quantity
GI Epithelial Cell Defined Culture Medium Kit: Basal medium and growth supplement, sold together and packaged separately. Supplement includes antibiotics. Store at -20C. Once thawed, mix the BM and GS, store at 4C, and use within 4 weeks. Size: Yields 50 ml CAT.#: 716DCK-50 Price: $75.00
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TEER Assay GI Epithelial Cell Medium : All-in-one ready-to-use optimized media for studying GI epithelial barrier function. Store at -20C. Once thawed, store at 4C and use within 4 weeks. Size: 50 ml CAT.#: 716TA-50 Price: $65.00
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Resources/Documents

Cell Apps Flyer Epithelial Cells

Format: PDF

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5 Important Cell Culture Rules

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

Downoad Now