Instructions HC-OA
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Chondrocytes Osteoarthritis (HC-OA) are derived from human articular cartilage of donors with OA.  Osteoarthritis is an inflammatory disease characterized by increased degradation of cartilage tissue in the joint due to overproduction of enzymes degrading the extracellular matrix.  Despite the initial proliferation and activation of chondrocytes, they are not able to efficiently repair the degrading cartilage.  Instead, chondrocytes undergo terminal differentiation and eventually apoptose, leading to mineralization of cartilage in a process resembling bone formation during development.  Thus, HC-OA provide a useful model to study changes in chondrocyte biology in response to abnormal environment of the OA joint.
HC-OA from Cell Applications, Inc. have been used to demonstrate that THF-alpha activates transcription of ADAMTS-4, a metalloproteinase implicated in cartilage degradation in OA, via p38-MAPK-dependent mechanism.
 
Characterization: Positive for aggrecan after differentiation
Human Chondrocytes-Osteoarthritis: HC-OA, Cartilage producing cells isolated from the joints of diseased tissue donors.

Details

Tissue
Human articular cartilage from donor w/ osteoarthritis
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HC-OA (1st passage) frozen in Basal Medium w/10% FBS, 10% DMSO
Kit
Cryovial frozen HC-OA (402OA-05), Growth Medium (411-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HC-OA

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HC-OA Total Kit: 5x10^5 Cells (from donor with Osteoarthritis, Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 402OAK-05a Price: $892.99
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Cryopreserved HC-OA, adult: Frozen HC from donor with Osteoarthritis (5x10^5) Size: 1 Cryovial CAT.#: 402OA-05a Price: $747.99
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Proliferating HC-OA, adult: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: 403OA-25a Price: $747.99
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Proliferating HC-OA, adult: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: 403OA-75a Price: $952.48
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Proliferating HC-OA, adult: Actively growing, dividing cells, in medium Size: 6 Well CAT.#: 403OA-6Wa Price: $877.14
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Proliferating HC-OA, adult: Actively growing, dividing cells, in medium Size: 96 well CAT.#: 403OA-96Wa Price: $1,006.29
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Related Products

Product Size CAT.# Price Quantity
Human Chondrocyte Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 410-500 Price: $53.81
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Human Chondrocyte Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: 411D-250 Price: $95.79
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Human Chondrocyte Differentiation Medium: Promotes cells to change from one type to another, more specialized, without Phenol Red Size: 250 ml CAT.#: 411DPR-250 Price: $110.70
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Human Chondrocyte Growth Medium: Growth medium without phenol red Size: 500 ml CAT.#: 411PR-500 Price: $118.39
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Human Chondrocyte Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 411-500 Price: $102.24
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Human Chondrocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 411K-500 Price: $110.85
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Human Chondrocyte Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 411-GS Price: $57.04
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Extended Family Products

Product Size CAT.# Price Quantity
Anti-MMP-1: Mouse MMP-1 Antibody Size: 100 ul CAT.#: CP10422 Price: $302.38
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Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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CTGF: Human Connective Tissue Growth Factor Size: 20 ug CAT.#: RP1060-20 Price: $193.73
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CTGF: Human Connective Tissue Growth Factor Size: 100 ug CAT.#: RP1060-100 Price: $484.31
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CTGF: Human Connective Tissue Growth Factor Size: 1000 ug CAT.#: RP1060-1000 Price: $4,089.75
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF402K Price: $430.50
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Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF402KS Price: $53.76
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MMP-1 ELISA Kit, Human: Human Matrix Metalloproteinase-1 ELISA Kit Size: 96 Wells CAT.#: CL0458 Price: $495.08
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Skeletal System Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

2017

Rosenberg, J.H., Rai, V., Dilisio, M.F., Sekundiak, T.D. and Agrawal, D.K., 2017. Increased expression of damage-associated molecular patterns (DAMPs) in osteoarthritis of human knee joint compared to hip joint. Molecular and Cellular Biochemistry, pp.1-11.

Dou, P., R. Hu, W. Zhu, Q. Tang, D Li, H. Li and W. Wang. 2017. Long non-coding RNA HOTAIR promotes expression of ADAMTS-5 in human osteoarthritic articular chondrocytes. Die Pharmazie, 72:113-117.
2016
Dua, R., K. Comella, R. Butler, G. Castellanos, B. Brazille, A. Claude, A. Agarwal, J. Liao and S. Ramaswamy. 2016. Integration of Stem Cell to Chondrocyte-Derived Cartilage Matrix in Healthy and Osteoarthritic States in the Presence of Hydroxyapatite NanoparticlesPLoS ONE, 11(2): e0149121.
Kong, Y., W. Wang, C. Zhang, Y. Wu, Y. Liu and X. Zhou. 2016. Cannabinoid WIN‑55,212‑2 mesylate inhibits ADAMTS‑4 activity in human osteoarthritic articular chondrocytes by inhibiting expression of syndecan‑1. Molecular Medicine Reports, 13:45669-4576
Samavedi, S., P. Diaz-Rodriguez, J. Erndt-Marino and M. Hahn. Tissue Eng Part A. 2016. A 3D chondrocyte-macrophage co-culture system to probe inflammation in experimental osteoarthritis. Tissue Engineering, Part A, doi:10.1089/ten.TEA.2016.0007.
2014
Song, J., C. Ahn, C. Chun and E. Jin. 2014. A long non-coding RNA, GAS5, plays a critical role in the regulation of miR-21 during osteoarthritis. J Orthopaedic Res, 32:1628-1635
2013
Xue, J., J. Wang, Q. Liu, and A. Luo. 2013. Tumor necrosis factor-α induces ADAMTS-4 expression in human osteoarthritis chondrocytes. Molecular medicine reports: 10.3892/mmr.2013.1729.
2005

Quintavalla, J., C. Kumar, S. Daouti, E. Slosberg, and S. Uziel-Fusi. 2005. Chondrocyte cluster formation in agarose cultures as a functional assay to identify genes expressed in osteoarthritis. Journal of cellular physiology. 204:560-566.