MSDS Cryopreserved Cells
Instructions HCF
Cell Apps Flyer Cardiovascular Cells
5 Important Cell Culture Rules
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Human Cardiac Fibroblasts (HCF) are the most prevalent cell type in the heart.  HCF from Cell Applications, Inc. provide an excellent model system to study many aspects of human heart function and pathophysiology.
HCF from Cell Applications, Inc. have been utilized in a numerous published studies, for example to:
  • Determine that electrical coupling between cardiomyocytes and fibroblasts is mediated by Ca2+-activated K+ channels that can be stimulated by estrogen receptor agonists
  • Show that antimitogenic effects of estradiol on HCF growth are mediated by cytochromes and metabolites
  • Demonstrate that in response to mechanical stretch, cardiac fibroblasts release TGF-β that downregulates trombomodulin, increases thromboembolism and induces cardiac fibroblast differentiation into myofibroblasts
  • Indicate that activation of G protein-coupled receptor kinase-2 prevents normal regulation of collagen synthesis in cardiac fibroblasts mimicking heart failure phenotype
  • Identify FGF2 signaling pathway as potential target for modulating apoptosis in cardiac pathology
  • Investigate the roles of scleraxis and AMPKα1 in scar formation following myocardial infarction
  • Show that the KATP channel opener KMUP-3 preserved cardiac function after myocardial infarction by enhancing the expression of NO synthase and restoring MMP-9/TIMP-1 balance
(Click to Enlarge) Human Cardiac Fibroblasts: HCF (L).  HCF immunolabeled for FSP (green), nuclei are stained with PI (R).

Details

Tissue
Normal healthy human heart tissue
QC
No bacteria, yeast, fungi, mycoplasma, virus
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 HCF (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen HCF (306-05f), Growth Medium (316-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 8
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions HCF

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved HCF, Adult: 5x10^5 Cells (Adult), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 306K-05a Price: $784.00
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Cryopreserved HCF, Adult: Frozen HCF (5x10^5) Size: 1 Cryovial CAT.#: 306-05a Price: $640.00
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Cryopreserved HCF, Fetal: Frozen HCF (5x10^5) Size: 1 Cryovial CAT.#: 306-05f Price: $640.00
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Cryopreserved HCF, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 306K-05f Price: $784.00
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Proliferating HCF, Adult: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 307-25a Price: $640.00
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Proliferating HCF, Adult: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 307-75a Price: $845.00
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Proliferating HCF, Adult: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 307-24Wa Price: $845.00
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Proliferating HCF, Adult: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 307-96Wa Price: $974.00
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Proliferating HCF, Fetal: Actively growing, dividing cells in medium Size: T-25 Flask CAT.#: 307-25f Price: $640.00
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Proliferating HCF, Fetal: Actively growing, dividing cells in medium Size: T-75 Flask CAT.#: 307-75f Price: $845.00
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Proliferating HCF, Fetal: Actively growing, dividing cells in medium Size: 24 Well CAT.#: 307-24Wf Price: $845.00
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Proliferating HCF, Fetal: Actively growing, dividing cells in medium Size: 96 Well CAT.#: 307-96Wf Price: $974.00
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Related Products

Product Size CAT.# Price Quantity
HCF Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 315-500 Price: $57.00
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HCF Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: 316-500 Price: $99.00
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HCF Growth Medium Kit: Basal Medium and Growth Supplement sold together, bottled separately Size: yields 500 ml CAT.#: 316K-500 Price: $108.00
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HCF Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: 316-GS Price: $51.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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Cytofect™ Fibroblast Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF103KS Price: $54.00
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Cytofect™ Fibroblast Transfection Kit: 250 x 24-Well Rxns Size: 1 Kit CAT.#: TF103K Price: $404.00
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HCF RNA, Adult: Total RNA prepared from Human Cardiac Fibroblasts, adult Size: 10 ug CAT.#: 306-R10a Price: $409.00
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HCF RNA, Adult: Total RNA prepared from Human Cardiac Fibroblasts, adult Size: 25 ug CAT.#: 306-R25a Price: $818.00
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HCF RNA, Fetal: Total RNA prepared from Human Cardiac Fibroblasts, fetal Size: 10 ug CAT.#: 306-R10f Price: $350.00
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HCF RNA, Fetal: Total RNA prepared from Human Cardiac Fibroblasts, fetal Size: 25 ug CAT.#: 306-R25f Price: $700.00
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Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free Size: 10 ug CAT.#: RP1028AF-10 Price: $89.00
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Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free Size: 100 ug CAT.#: RP1028AF-100 Price: $347.00
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Human FGF-147 basic, Animal-Free: Human Fibroblast Growth Factor-basic-147, Animal-Free Size: 1000 ug CAT.#: RP1028AF-1000 Price: $1,717.00
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Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free Size: 1000 ug CAT.#: RP1146AF-1000 Price: $1,717.00
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Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free Size: 10 ug CAT.#: RP1146AF-10 Price: $89.00
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Human FGF-basic 154, Animal-Free: Human Fibroblast Growth Factor-basic-154, Animal-Free Size: 100 ug CAT.#: RP1146AF-100 Price: $276.00
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Human FGF9 ELISA Kit: Human Fibroblast Growth Factor 9 ELISA Kit Size: 96 Wells CAT.#: CL0348 Price: $457.00
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Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic Size: 10 ug CAT.#: RP1028-10 Price: $81.00
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Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic Size: 100 ug CAT.#: RP1028-100 Price: $317.00
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Human Fibroblast Growth Factor 147 Basic (FGF-147 basic/FGF-2): Human Fibroblast Growth Factor-147-basic Size: 1000 ug CAT.#: RP1028-1000 Price: $1,561.00
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Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic Size: 1000 ug CAT.#: RP1146-1000 Price: $1,561.00
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Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic Size: 10 ug CAT.#: RP1146-10 Price: $81.00
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Human Fibroblast Growth Factor 154 Basic (FGF-basic 154 / FGF-2): Human Fibroblast Growth Factor-154-basic Size: 100 ug CAT.#: RP1146-100 Price: $253.00
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Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic Size: 10 ug CAT.#: RP1029-10 Price: $81.00
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Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic Size: 50 ug CAT.#: RP1029-50 Price: $194.00
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Human Fibroblast Growth Factor Acidic (FGF-1): Human Fibroblast Growth Factor-acidic Size: 1000 ug CAT.#: RP1029-1000 Price: $1,561.00
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Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 Size: 20 ug CAT.#: RP1148-20 Price: $194.00
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Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 Size: 100 ug CAT.#: RP1148-100 Price: $484.00
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Human Fibroblast Growth Factor-21 (FGF-21): Human Fibroblast Growth Factor-21 Size: 1000 ug CAT.#: RP1148-1000 Price: $3,175.00
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Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 Size: 20 ug CAT.#: RP1068-20 Price: $194.00
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Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 Size: 100 ug CAT.#: RP1068-100 Price: $624.00
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Human Fibroblast Growth Factor-22 (FGF-22): Human Fibroblast Growth Factor-22 Size: 1000 ug CAT.#: RP1068-1000 Price: $5,166.00
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Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 Size: 25 ug CAT.#: RP1031-25 Price: $194.00
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Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 Size: 100 ug CAT.#: RP1031-100 Price: $484.00
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Human Fibroblast Growth Factor-4 (FGF-4): Human Fibroblast Growth Factor-4 Size: 1000 ug CAT.#: RP1031-1000 Price: $3,175.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 25 ug CAT.#: RP1127-25 Price: $194.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 100 ug CAT.#: RP1127-100 Price: $484.00
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Human Gamma-Interferon Inducible Protein 10 (IP-10 / CXCL10): Human gamma-Interferon Inducible Protein 10 Size: 1000 ug CAT.#: RP1127-1000 Price: $3,175.00
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Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 20 ug CAT.#: RP1008AF-20 Price: $213.00
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Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 100 ug CAT.#: RP1008AF-100 Price: $533.00
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Human GM-CSF, Animal-Free: Human Granulocyte Macrophage-Colony Stimulating Factor, Animal-Free Size: 1000 ug CAT.#: RP1008AF-1000 Price: $3,788.00
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Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 20 ug CAT.#: RP1008-20 Price: $194.00
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Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 100 ug CAT.#: RP1008-100 Price: $484.00
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Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF): Human Granulocyte Macrophage-Colony Stimulating Factor Size: 1000 ug CAT.#: RP1008-1000 Price: $3,444.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 50 ug CAT.#: 1H30-50 Price: $139.00
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Human Heart RNA: Total RNA prepared from human heart tissue Size: 250 ug CAT.#: 1H30-250 Price: $522.00
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Human MIF ELISA Kit: Human Macrophage Migration Inhibitory Factor ELISA Kit Size: 96 wells CAT.#: CL0813 Price: $511.00
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Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor Size: 25 ug CAT.#: RP1153-25 Price: $194.00
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Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor Size: 100 ug CAT.#: RP1153-100 Price: $484.00
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Human Migration Inhibitory Factor (MIF): Human Migration Inhibitory Factor Size: 1000 ug CAT.#: RP1153-1000 Price: $3,175.00
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Mouse Fibroblast Growth Factor Receptor 1 Antibody: Mouse Fibroblast Growth Factor Receptor 1 Antibody Size: 100 ul CAT.#: CP10102 Price: $333.00
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Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody: Mouse Fibroblast Growth Factor Receptor Tyrosine Kinase 4 Antibody Size: 100 ul CAT.#: CP10103 Price: $333.00
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Polyclonal Fibroblast Growth Factor 2 Rabbit Antibody: Polyclonal Fibroblast Growth Factor 2, FGF2 (bFGF), Rabbit Antibody Size: 100 ul CAT.#: CA0259 Price: $302.00
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Polyclonal Fibroblast Growth Factor 7, KGF, Rabbit Antibody: Polyclonal Fibroblast Growth Factor 7, KGF, Rabbit Antibody Size: 100 ul CAT.#: CA0853 Price: $302.00
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Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody: Polyclonal Fibroblast Growth Factor-Receptor 2 Antibody Size: 100 ul CAT.#: CB4365 Price: $333.00
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Rabbit Fibroblast Growth Factor 8 Antibody: Rabbit Fibroblast Growth Factor 8 Antibody Size: 100 ul CAT.#: CA1216 Price: $302.00
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Rabbit Macrophage Migration Inhibitory Factor Antibody: Rabbit Macrophage Migration Inhibitory Factor Antibody Size: 100 ul CAT.#: CA1052 Price: $302.00
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $55.00
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Resources/Documents

Cell Apps Flyer Cardiovascular Cells

Format: PDF

Downoad Now
5 Important Cell Culture Rules

Format: PDF

Downoad Now
Cell Apps Poster Primary Cells

Format: PDF

Downoad Now
Cell Applications Inc Brochure

Format: PDF

Downoad Now

Publications

2017
Ross, G., T. Bajwa, S. Edwards, L. Emelyanova, F. Rivi, E. Holmuhamedov, P. Werner, F. Downey, A. Tajik and A. Jahangir. 2017. Enhanced store-operated Ca2+ influx and ORAI1 expression in ventricular fibroblasts from human failing heart. Biology Open Advance article
2016
Bagchi, R.., R. Wang, F. Jahan, J. Wigle and M. Czubryt. 2016. Regulation of scleraxis transcriptional activity by serine phosphorylation. J Molec & Cell Cardiol, doi:10.1016/j.yjmcc.2016.02.013.
Czubryt, M. 2016. Inhibition of collagen synthesis. Patent US 9238685 B2. 
Hookway, T., J. Butts, E. Lee, H. Tang and T. McDevitt. 2016. Aggregate formation and suspension culture of human pluripotent stem cells and differentiated progenyMethods, 101:11-20.
Hung, C., C. Chou, C. Liao, Y. Lin, X. Wu, Y. Chang, Y. Chen, V. Wu, M. Su, Y. Ho, M. Chen, K. Wu and Y. Lin. 2016. Aldosterone Induces Tissue Inhibitor of Metalloproteinases-1 Expression and Further Contributes to Collagen Accumulation. Hypertension, 67:1309-1320.
Jonsson, M., R. Hartman, M. Ackers-Johnson, W. Tan, B. Lim, T. van Veen and R. Foo. 2016. A Transcriptomic and Epigenomic Comparison of Fetal and Adult Human Cardiac Fibroblasts Reveals Novel Key Transcription Factors in Adult Cardiac Fibroblasts. JACC: Basic to Translational Science, 7:590-602.
Nagpal, V., R. Rai, A. Place, S. Murphy, S. Verma, A. Ghosh and D. Vaughan. 2016. MiR-125b Is Critical for Fibroblast-to-Myofibroblast Transition and Cardiac Fibrosis. Circulation, 133:291-301.
Rizvi, F., A. DeFranco, R. Siddiqui, U. Negmadjanov, L. Emelyanova, A. Holmuhamedov, G. Ross, Y. Shi, E. Holmuhamedov, D. Kress, A. Tajik and A. Jahangir. 2016. Chamber-specific differences in human cardiac fibroblast proliferation and responsiveness toward simvastatinAm J Physiol – Cell Physiol, 311:C330-339.
Sundaresan, N., S. Bindu, V. Pillai, S. Samant, Y. Pan, J. Huang, M. Gupta, R. Nagalingam, D. Wolfgeher, E. Verdin and M. Gupta. 2016. SIRT3 Blocks Aging-Associated Tissue Fibrosis in Mice by Deacetylating and Activating Glycogen Synthase Kinase 3β. Mol Cell Biol, 36:678-692.
2015
Momtahan, N., N. Poornejad, J. Struk, A. Castleton, B. Herrod, B. Vance, J. Eatough, D. Roeder, P. Reynolds, and D. Cook. 2015. Automation of Pressure Control Improves Whole Porcine Heart Decellularization. Tissue Engineering Part C: Methods, doi:10.1089/ten.TEC.2014.0709.
Philip, J., A. Razzaque, M. Han, J. Li, T. Thecanat, X. Xu, and S. Akhter. 2015. Regulation of Mitochondrial Oxidative Stress by β-arrestins in Cardiac Fibroblasts.  Disease Models & Mechanisms. doi: 10.1242/dmm.019968.
Tomita, K., M. Takashina, N. Mizuno, K. Sakata, K. Hattori, J. Imura, W. Ohashi, and Y. Hattori. 2015. Cardiac fibroblasts: contributory role in septic cardiac dysfunction. Journal of Surgical Research, 193:874–887.
2014
Ichiki, T., J. Schirger, B. Huntley, F. Brozovich, J. Maleszewski, S. Sandberg, S. Sangaralingham, S. Park, and J. Burnett. 2014. Cardiac fibrosis in end-stage human heart failure and the cardiac natriuretic peptide guanylyl cyclase system: Regulation and therapeutic implications. J Molecular and Cellular Cardiol, 75:199-205.
Li, J., J. Philip, X. Xu, T. Theccanat, M. Razzaque, and S. Akhter. 2014. β-Arrestins regulate human cardiac fibroblast transformation and collagen synthesis in adverse ventricular remodeling. JMCC, 76:73-83.
Noppe, G., C. Dufeys, P. Buchlin, N. Marquet, D. Castanares-Zapatero, M. Balteau, N. Hermida, C. Bouzin, H. Esfahani, B. Viollet, L. Bertrand, J. Balligand, J. Vanoverschelde, C. Beauloye, and S. Horman. 2014. Reduced scar maturation and contractility lead to exaggerated left ventricular dilation after myocardial infarction in mice lacking AMPKα1. Journal of Molecular and Cellular Cardiology, 74:32–43.
2013
Yue, Z., Y. Zhang, J. Xie, J. Jiang, and L. Yue. 2013. Transient Receptor Potential (TRP) channels and cardiac fibrosis. Current topics in medicinal chemistry. 13:270-282
2012
Bagchi, R.A., and M.P. Czubryt. 2012. Synergistic roles of scleraxis and Smads in the regulation of collagen 1α2 gene expression. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1823:1936-1944.
Czubryt, M. 2012. Inhibition of collagen synthesis. Patent Application US 20140288003 A1.
Savvatis, K., S. van Linthout, K. Miteva, K. Pappritz, D. Westermann, J.C. Schefold, G. Fusch, A. Weithäuser, U. Rauch, P.-M. Becher, K. Klingel, J. Ringe, A. Kurtz, H.-P. Schultheiss, and C. Tschöpe. 2012. Mesenchymal Stromal Cells but Not Cardiac Fibroblasts Exert Beneficial Systemic Immunomodulatory Effects in Experimental Myocarditis. PloS one. 7:e41047.
2011
Bogaard, H.J., S. Mizuno, A.A.A. Hussaini, S. Toldo, A. Abbate, D. Kraskauskas, M. Kasper, R. Natarajan, and N.F. Voelkel. 2011. Suppression of Histone Deacetylases Worsens Right Ventricular Dysfunction after Pulmonary Artery Banding in Rats. American journal of respiratory and critical care medicine. 183:1402-1410.
Chen, I. 2011. Use of kmup-3 for myocardial infarction. Patent Application US 20120095013 A1.
Czubryt, M. 2011. Modulation of scleraxis using a dominant negative scleraxis mutant with a basic dna-binding domain deletion. Patent Application US 20130123198 A1.
D'Souza, K.M., R. Malhotra, J.L. Philip, M.L. Staron, T. Theccanat, V. Jeevanandam, and S.A. Akhter. 2011. G Protein-coupled Receptor Kinase-2 Is a Novel Regulator of Collagen Synthesis in Adult Human Cardiac Fibroblasts. Journal of Biological Chemistry. 286:15507-15516.
Liu, C.P., J.L. Yeh, B.N. Wu, C.Y. Chai, I.J. Chen, and W.T. Lai. 2011. KMUP3 attenuates ventricular remodelling after myocardial infarction through eNOS enhancement and restoration of MMP9/TIMP1 balance. Brit. J. Pharmacol. 162:126-135.
Ma, X. 2011. Studies on signals mediating or preventing the intracrine induction of chromatin compaction and cell death by high molecular weight fibroblast growth factor 2. University of Manitoba, PhD dissertation.
Miteva, K., M. Haag, J. Peng, K. Savvatis, P.M. Becher, M. Seifert, K. Warstat, D. Westermann, J. Ringe, M. Sittinger, H.-P. Schultheiss, C. Tschöpe, and S. Van Linthout. 2011. Human Cardiac-Derived Adherent Proliferating Cells Reduce Murine Acute Coxsackievirus B3-Induced Myocarditis. PloS one. 6:e28513.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Methods of screening using a natriuretic peptide receptor. Patent US 7943296 B2.
Silberbach, M., C. Roberts, and N. Airhart. 2011. Natriuretic peptide related fragment in cardiovascular disease. Patent Application US 20110218230 A1.
2010
Peng, H., O.A. Carretero, E.L. Peterson, and N.-E. Rhaleb. 2010. Ac-SDKP inhibits transforming growth factor-β1-induced differentiation of human cardiac fibroblasts into myofibroblasts. American J. of Physiology 298:H1357-H1364.
2007
Wang, Y.-J., M.-W. Lin, S.-N. Wu, and R.J. Sung. 2007. The activation by estrogen receptor agonists of the BKCa-channel in human cardiac fibroblasts. Biochemical pharmacology. 73:1347-1357. Young, E.F., and E.E. Marcantonio. 2007. A Novel Subcellular Collagen Organization Process Visualized by Total Internal Reflection Fluorescence Microscopy. Cell Communication and Adhesion. 14:169-180.
2006
Wang, Y.-J., R. Sung, M.-W. Lin, and S.-N. Wu. 2006. Contribution of BKCa-Channel Activity in Human Cardiac Fibroblasts to Electrical Coupling of Cardiomyocytes-Fibroblasts. J Membrane Biol. 213:175-185.
2005

Dubey, R.K., E.K. Jackson, D.G. Gillespie, M. Rosselli, F. Barchiesi, A. Krust, H. Keller, L.C. Zacharia, and B. Imthurn. 2005. Cytochromes 1A1/1B1-and catechol-O-methyltransferase-derived metabolites mediate estradiol-induced antimitogenesis in human cardiac fibroblast. Journal of Clinical Endocrinology & Metabolism. 90:247-255.