(Click to Enlarge) Cell RNA (A) Human Fibroblast–Like Synoviocytes, Rheumatoid Arthritis (HFLS-RA) Total RNA. RNA was electrophoresed in 1% agarose gel to show the distinct 28S and 18S ribosomal RNA bands. RT-PCR was performed with Hsp60 primers and CD55 primers to produce 2.2 kb and 582 bp products. (B) Re-Differentiated Human Adult Chondrocytes (RD-HC) Total RNA. RNA was electrophoresed in 1% agarose gel to show the distinct 28S and 18S ribosomal RNA bands. RT-PCR was performed with Aggrecan and Hsp60 primers to produce 213 bp and 2.2 Kb products.
High quality total RNA from human and animal primary cells using the modified guanidium thiocyanate method. RNA is offered at 10 & 25 μg/vial at 1 mg/ml, and is ready for use in downstream applications like microarray hybridization, PCR cDNA synthesis and RT-PCR analysis.
See Also Tissue RNA
Quality control tests confirm RNA integrity and stability:
- Distinct 28S and 18S ribosomal RNA shown on 1% denaturing gel before and after 37oC incubation
- No visible Genomic DNA band of 23 kb detected
- No genomic DNA contamination detected following PCR amplification using house-keeping gene G3PDH primers
- A 2.2 kb product from mRNA was detected following RT-PCR amplification using human heat shock gene Hsp60 primers
- A discrete band of 2 kb was detected upon hybridization with a human beta-actin cDNA probe in Northern Blot analysis
- Primers were designed to detect mRNA for genes of interest to confirm cell phenotype
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