Instructions CnC
5 Important Cell Culture Rules
MSDS Cryopreserved Cells
Cell Apps Flyer Skeletal System Cells
Cell Apps Poster Primary Cells
Cell Applications Inc Brochure

Description

Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage.  Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice.
 
Canine Chondrocytes (CnC) from Cell Applications, Inc. were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III and trans-pterostilbene.  They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices.
Canine Chondrocytes: CnC.  Cartilage producing cells isolated from dog joint tissue.

Details

Tissue
Normal healthy canine articular cartilage
QC
No bacteria, yeast, fungi, mycoplasma
Bioassay
Attach, spread, proliferate in Growth Med
Cryovial
500,000 CnC (1st passage) frozen in Basal Medium w/ 10% FBS, 10% DMSO
Kit
Cryovial frozen CnC (Cn402-05), Growth Medium (Cn411-500), Subcltr Rgnt Kit (090K)
Proliferating
Shipped in Gr Med, 2nd psg (flasks or plates)
Doublings
At least 10
Applications
Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.
Instructions CnC

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MSDS Cryopreserved Cells

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Products

Product Size CAT.# Price Quantity
Cryopreserved CnC: Frozen CnC (5x10^5) Size: 1 Cryovial CAT.#: Cn402-05 Price: $694.18
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Cryopreserved CnC Total Kit: 5x10^5 Cells, Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: Cn402K-05 Price: $840.52
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Proliferating CnC: Actively growing, dividing cells, in medium Size: T-25 Flask CAT.#: Cn403-25 Price: $694.18
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Proliferating CnC: Actively growing, dividing cells, in medium Size: T-75 Flask CAT.#: Cn403-75 Price: $898.67
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Proliferating CnC: Actively growing, dividing cells, in medium Size: 6 well CAT.#: Cn403-6W Price: $898.67
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Proliferating CnC: Actively growing, dividing cells, in medium Size: 96 Well CAT.#: Cn403-96W Price: $1,027.82
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Related Products

Product Size CAT.# Price Quantity
Canine Chondrocyte Basal Medium: Without growth supplement (GS).  Add GS before use. Size: 500 ml CAT.#: Cn410-500 Price: $57.04
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Canine Chondrocyte Differentiation Medium: Promotes cells to change from one type to another, more specialized Size: 250 ml CAT.#: Cn411D-250 Price: $71.03
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Canine Chondrocyte Growth Medium: All-in-one ready-to-use Size: 500 ml CAT.#: Cn411-500 Price: $97.94
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Canine Chondrocyte Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: Cn411K-500 Price: $113.01
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Canine Chondrocyte Growth Supplement: Added to Basal Medium to create Growth Medium Size: 50 ml CAT.#: Cn411-GS Price: $50.58
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Extended Family Products

Product Size CAT.# Price Quantity
Cell Freezing Medium: For general cryopreservation of most primary cells. Contains FBS & DMSO. Size: 50 ml CAT.#: 040-50 Price: $53.81
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CnC RNA: Total RNA prepared from Canine Chondrocytes Size: 10 ug CAT.#: Cn402RD-R10 Price: $349.78
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CnC RNA: Total RNA prepared from Canine Chondrocytes Size: 25 ug CAT.#: Cn402RD-R25 Price: $699.56
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Cyto-X Colorimetric Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $138.84
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Cyto-X Colorimetric Cell Counting Reagent: 100 tests Size: Sample CAT.#: 028-S Price: $35.88
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Cytofect™ Chondrocyte Transfection Kit: 175 x 24-Well Rxns Size: 1 Kit CAT.#: TF402K Price: $430.50
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Cytofect™ Chondrocyte Transfection Kit: 25 x 24-Well Rxns Size: 1 Sample Kit CAT.#: TF402KS Price: $53.76
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Subculture Reagent Kit: 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution Size: 1 Kit CAT.#: 090K Price: $54.89
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Resources/Documents

5 Important Cell Culture Rules

Format: PDF

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Cell Apps Flyer Skeletal System Cells

Format: PDF

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Cell Apps Poster Primary Cells

Format: PDF

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Cell Applications Inc Brochure

Format: PDF

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Publications

. 2015
Martinez, S., Y. Chen, E. Ho, S. Martinez, and N. Davies. 2015. Pharmacological effects of a C-phycocyanin-based multicomponent nutraceutical in an in-vitro canine chondrocyte model of osteoarthritisCanadian J Vet Res, 79:241-249.
Yamka, R. and N. Frantz. 2015. Compositions including pyruvate for companion animals and methods of use thereofPatent US 8999375 B2.
2013
Shaik, J., J.S. Mohammed, M. McShane, and D. Mills. 2013a. In vitro evaluation of chondrosarcoma cells and canine chondrocytes on layer-by-layer (LbL) self-assembled multilayer nanofilms. Biofabrication. 5:015004.
Shaik, J., J. Shaikh Mohammed, M.J. McShane, and D.K. Mills. 2013b. Chondrocyte Behavior on Micropatterns Fabricated Using Layer-by-Layer Lift-Off: Morphological Analysis. Journal of Medical Engineering. 2013.
2012
Wright, L.D., K.D. McKeon-Fischer, Z. Cui, L.S. Nair, and J.W. Freeman. 2012. PDLA/PLLA and PDLA/PCL nanofibers with a chitosan-based hydrogel in composite scaffolds for tissue engineered cartilage. Journal of tissue engineering and regenerative medicine: 10.1002/term.1591
2010
Yamka, R. and N. Frantz. 2010. Compositions including pyruvate for companion animals and methods of use thereof. Patent Application US 20120289598 A1.
2008
Remsberg, C.M., J.A. Yáñez, Y. Ohgami, K.R. Vega-Villa, A.M. Rimando, and N.M. Davies. 2008. Pharmacometrics of pterostilbene: preclinical pharmacokinetics and metabolism, anticancer, antiinflammatory, antioxidant and analgesic activity. Phytotherapy Research. 22:169-179.
Yáñez, J.A., C.M. Remsberg, K.R. Vega-Villa, N.D. Miranda, J. Navas, Y. Ohgami, D. McCormick, K. Hughes, C. Temple, and S.A. Martinez. 2008. Pharmacological evaluation of Glyco-Flex® III and its constituents on canine chondrocytes. Journal of Medical Sciences. 8:1-14.