MSDS 027-05
MSDS 029-05
MSDS 120-25
MSDS 120-100
MSDS 122-20
MSDS 123-100
MSDS 124-25
MSDS 124-100
MSDS 125-50
MSDS 125-75
MSDS 125-100
MSDS 126-2.5
MSDS 126-100
MSDS 126XF-100
MSDS 127-2.5

Description

Cells adhere to one another and to the extracellular matrix durinig normal physiology and development, but also in pathological conditions such as chronic inflammation and tumor metastasis. 
 
For example, during extravasation from blood vessels into the tissue space, leukocytes first weakly adhere to and roll upon vascular endothelial cells via cell surface receptors known as selectins.  Subsequent, stronger adhesion ensues using other receptors and ligands, such as LFA-1 and ICAM-1.  Transendothelial migration follows, where the leukocytes squeeze between the tight endothelial cell junctions.  As a final impediment to entering the tissue space, the leukocytes encounter the basement membrane, a mixture of extracellular components that ensheathes the vessel. The leukocytes utilize still other receptors to bind ECM, and also secrete matrix-degrading enzymes to facilitate their passage.  Unfortunately, tumor cells utilze a similar mechanism as they spread to distant parts of the body during metastasis. 
 
Organs and tissues of the body also contain extensive networks of extracellular matrix, acting as structural scaffolding and regulating cell adhesion, migration and behavior.  Finally certain cells, when cultured in vitro, behave more robustly when adhering to a cell culture vessel coated with an ECM analog, component or other attachment factor.  Thus, the study of cell adhesion remains an area of particular interest to cell biologists.
(Click to Enlarge) Attachment / Matrix Solutions. Depiction of leukocyte adhesion to surface receptors on vascular endothelial cells (L).  Collagen Strand (R).

Details

Attachment Factor Solution
Components:Bovine Gelatin
Cell Types:Various, especially Endothelial Cells (Required for microvascular ECs)
Applications:Promotes attachment and spreading of endothelial cells
Collagen Coated T-75 Flask
Components:Bovine Collagen
Cell Types:Various, including HFDPC
Applications:For attachment and spreading of cells
Collagen Coating Solution
Components:Bovine Collagen
Cell Types:Various
Applications:For coating plates, flasks and dishes to promote cell attachment
EHS Matrix Extract (with and w/out Phenol Red)
ComponenetsHeterogeneous mix of extracellular matrix / basement membrane components: Laminin, entactin, collagen, HSPG, growth factors extracted from mouse Engelbreth-Holm-Swarm tumor
Cell Types:Various
Applications:For culturing cells where adhesion is dependent upon ECM-coated surface
Extracellular Matrix Attachment Factor Solution
Components:Bovine Fibronectin & Gelatin
Cell Types:Various, especially Endothelial Cells
Applications:Promotes cell attachment & spreading. Prevents endothelial cell monolayer from peeling under shear stress.
Extracellular Matrix Protein Coated T-25 flasks
Components:Bovine Fibronectin & Gelatin
Cell Types:Various, especially Endothelial Cells
Applications:For culturing cells where adhesion is dependent upon ECM-coated surface
Neuron Coating Solution I
Components:Poly-D-Lysine
Cell Types:Neurons
Applications:For the coating of cell cultureware to enhance adhesion
Neuron Coating Solution II
Components:Poly-D-Lysine
Cell Types:Neurons, especially RDRGN & HBN
Applications:For the coating of cell cultureware to enhance adhesion
Poly-D-Lysine & Laminin
Components:Poly-D-Lysine, Mouse Laminin
Cell Types:Various
Applications:For culturing cells where adhesion is dependent upon ECM-coated surface
Rat Tail Collagen Coating Solution
Components:Rat Tail Collagen
Cell Types:Various, especially Endothelial Cells, Hepatocytes, Muscle Cells
Applications:For coating plates, flasks and dishes to promote cell attachment
Rat Tail Collagen Solution
Components:Rat Tail Collagen
Cell Types:Various, especially Endothelial Cells, Hepatocytes, Muscle Cells
Applications:Enhances cell attachment and proliferation, or promotion of cell-specific morphology and function
 
Need help choosing?  See our Media Selection Guide

Products

Product Size CAT.# Price Quantity
Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin Size: 100 ml CAT.#: 123-100 Price: $19.00
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Attachment Factor Solution (AFS): AFS, contains Bovine Gelatin Size: 500 ml CAT.#: 123-500 Price: $73.00
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Bovine Collagen Coating Solution: Bovine Collagen Size: 50 ml CAT.#: 125-50 Price: $38.00
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Bovine Collagen Coating Solution: Bovine Collagen Size: 100 ml CAT.#: 125-100 Price: $75.00
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Collagen Coated T-75 Flask: Bovine Collagen Size: 1 Flask CAT.#: 125-75 Price: $27.00
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ECM Attachment Factor Solution: ECM AFS, contains Bovine Fibronectin & Gelatin Size: 25 ml CAT.#: 120-25 Price: $54.00
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ECM Attachment Factor Solution: ECM AFS, contains Bovine Fibronectin & Gelatin Size: 100 ml CAT.#: 120-100 Price: $194.00
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ECM Protein Coated T-25 Flasks: ECM attachment factor Size: 2 Flasks CAT.#: 121-25-02 Price: $30.00
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ECM Protein Coated T-25 Flasks: ECM attachment factor Size: 10 Flasks CAT.#: 121-25-10 Price: $118.00
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EHS Matrix Extract: Basement membrane components extracted from Engelbreth-Holm-Swarm (EHS) mouse tumor Size: 2.5 ml CAT.#: 126-2.5 Price: $123.00
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EHS Matrix Extract wo Phenol Red: EHS Matrix Extract concentrate without phenol red Size: 2.5 ml CAT.#: 126PR-2.5 Price: $133.00
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HiPSC Coating Solution: EHS Matrix Extract, pre-diluted and ready-to-use for coating tissue culture ware Size: 100 ml CAT.#: 126-100 Price: $50.00
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HiPSC Coating Solution, Xeno-Free: Truncated Vitronectin, no animal components, pre-diluted and ready-to-use for coating tissue culture ware Size: 100 ml CAT.#: 126xf-100 Price: $152.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 10 ml CAT.#: 027-10 Price: $31.00
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Neuron Coating Solution I: Poly-D-Lysine coating for neurons Size: 5 ml CAT.#: 027-05 Price: $16.00
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Neuron Coating Solution II: Poly-D-Lysine coating for neurons, especially RDGRN and HBN. Size: 5 ml CAT.#: 029-05 Price: $27.00
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Poly-D-Lysine & Laminin: PDL & mouse laminin, for cell attachment Size: 2.5 ml CAT.#: 127-2.5 Price: $70.00
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Rat Tail Collagen: Type I Collagen Size: 25 ml CAT.#: 124-25 Price: $113.00
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Rat Tail Collagen: Type I Collagen Size: 100 ml CAT.#: 124-100 Price: $425.00
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Rat Tail Collagen Coating Solution: Type I Collagen Size: 20 ml CAT.#: 122-20 Price: $27.00
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Resources/Documents

Publications

2017
Meyers, M., J. Rink, Q. Jiang, M. Kelly, J. Vercammen, C. Thaxton and M. Kibbe. 2017. Systemically administered collagentargeted gold nanoparticles bind to arterial injury following vascular interventions. Physiological Reports, 5:e13128.
2016
Suzuki, T., T. Aoyama, N. Suzuki, M. Kobayashi, T. Fukami, Y. Matsumoto and K. Tomono. 2016. Involvement of a proton-coupled organic cation antiporter in the blood–brain barrier transport of amantadine. Biopharmaceutics & Drug Disposition, 37:323-335.
2015
Colacino, K., C. Arena, S. Dong, M. Roman, R. Davalos and Y. Lee. 2015. Folate Conjugated Cellulose Nanocrystals Potentiate Irreversible Electroporation-induced Cytotoxicity for the Selective Treatment of Cancer Cells. Technol Cancer Res Treat, 14:757-766.
González-Miguel, J., R. Morchón, E. Carretón, J. Montoya-Alonso and F. Simón. 2015. Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease endarteritis? Parasites & Vectors, 8:194.
Kim, S., E. Choi, H. Lee, C. Moon, and E. Kim. 2015. Transthyretin as a new transporter of nanoparticles for receptor-mediated transcytosis in rat brain microvessels. Colloids and Surfaces B: Biointerfaces, 136:989-996.
2014
Camós, S., C. Gubern, M. Sobrado, R. Rodriguez, V. Romera, M. Moro, I. Lizasoain, J. Serena, J. Mallolas and M. Castellanos. 2014. The high-mobility group I-Y transcription factor is involved in cerebral ischemia and modulates the expression of angiogenic proteins. Neuroscience, 269:112-130.
Carver, K., D. Lourim, A. Tryba and D. Harder. 2014. Rhythmic expression of cytochrome P450 epoxygenases CYP4x1 and CYP2c11 in the rat brain and vasculature. Am J Physiol – Cell Physiol, 307:C989-C998.
Quinn, M., M. McMillin, C. Galindo, G. Frampton, H. Pae and S. DeMorrow. 2014. Bile acids permeabilize the blood brain barrier after bile duct ligation in rats via Rac1-dependent mechanisms. Digestive and Liver Disease, 46:527-534.

Uzer, G., S. Pongkitwitoon, C. Ian, W. Thompson, J. Rubin, M. Chan, and S. Judex. 2014. Gap junctional communication in osteocytes is amplified by Low intensity vibrations in vitro. PLoS ONE, March 10.

2012

DeCoster, M., J. McNamara, K. Cotton, D. Green, C. Jeyasankar, R. Idowu, K. Evans, Z. Xing, and Y. Lvov. Bionanocomposites for multidimensional Brain Cell signaling. Ch. 8. In Thomas, S., N. Ninan, S. Mohan, and E. Francis. 2012. Natural Polymers, Biopolymers, Biomaterials, Composites, Blends, and IPNS. Advances in Materials Science, Volume 2.
Liu, B. 2012. The role of GRK2 in hypertension and regulation of GPR30. MS Thesis, University of Western Ontario.
Naughton, G., J. Mansbridge, R. Pinney, and J. Zeltinger. 2012. Methods for using a three-dimensional stromal tissue to promote angiogenesis.  Patent US 8128924 B2.
Uzer, G. 2012. Role of Fluid Shear Modulation on Bone Cell Metabolism during High- Frequency Oscillatory Vibrations. PhD Dissertation, Stony Brook U.
2010
Sheikh-Ali, M., S. Sultan, A.-R. Alamir, M.J. Haas, and A.D. Mooradian. 2010a. Effects of antioxidants on glucose-induced oxidative stress and endoplasmic reticulum stress in endothelial cells. Diabetes research and clinical practice. 87:161-166.
Sheikh-Ali, M., S. Sultan, A.-R. Alamir, M.J. Haas, and A.D. Mooradian. 2010b. Hyperglycemia-induced endoplasmic reticulum stress in endothelial cells. Nutrition. 26:1146-1150.
2009
Hendriks-Balk, M., J. Unen, H. Burt, and J. Butler. 2009. Extracellular S1P disappearance: effect on signalling potency. Source: Balk, M. 2009. Regulation of cardiovascular GPCR signaling. PhD Thesis, U Amsterdam.