Description

The 3D Skin Model is a highly physiological, three-dimensional cellular system of Human Epidermal Keratinocytes (HEK) for in vitro studies, offering an excellent tool to examine aspects of epithelial function and disease, particularly those related to skin biology and toxicology. 
Basic science and pharmaceutical researchers use the 3D Skin Model to replace animal tests for the assessment of skin irritation due to chemicals, skin-cream-based drugs and cosmetics. CAI's Skin Model can also help avoid misclassification of chemicals and skin corrosion observed in animal systems (1, 2). Other applications include phototoxicity, percutaneous absorption & penetration, wound healing and metabolism.  The cells are grown and differentiated into a stratified squamous epithelium on PCF inserts with a liquid/air interface. Contact us for more information.
  1. Eun & Nam, Exog Dermatol, 2:1–5 (2003)
  2. York et al., Contact Dermatitis, 34:204 (1996)

(Click to Enlarge)  A. Depiction of Human Skin.  B. Human Epidermal Keratinocytes (HEK) at ~80% confluency.  C. Crystal Violet Staining.  D. Hematoxylin & Eosin Staining of HEK differentiated into stratified squamous epithelium after 14 days.

Details

The Skin Model (domestic US) consists of HEK differentiated into stratified squamous epithelium, whereas the Kit (for international customers) contains Cryopreserved HEK, media, PCF inserts, reagents, subculture kit, and detailed instructions to form 3D Skin Model.

Products

Product Size CAT.# Price Quantity
3-D Skin Model: Cells, Media, Reagents & Inserts (See Details tab for specifics) Size: 1 Kit CAT.#: 102K-3D Price: $640.00
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3-D Skin Model: HEK differentiated into stratified squamous epithelium on PCF inserts Size: 24 Inserts CAT.#: 102-3D-24 Price: $1,184.00
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3-D Skin Model: HEK differentiated into stratified squamous epithelium on PCF inserts Size: 12 Inserts CAT.#: 102-3D-12 Price: $640.00
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Related Products

Product Size CAT.# Price Quantity
Cryopreserved HEK, Fetal: Frozen HEK (5x10^5) Size: 1 Cryovial CAT.#: 102-05f Price: $414.00
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Cryopreserved HEK, Fetal: 5x10^5 Cells (Fetal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 102K-05f Price: $563.00
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Cryopreserved HEK, Neonatal: 5x10^5 Cells (Neonatal), Medium & Subculture Reagents (See Details tab for specifics) Size: 1 Kit CAT.#: 102K-05n Price: $576.00
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Cryopreserved HEK, Neonatal: Frozen HEK (5x10^5) Size: 1 Cryovial CAT.#: 102-05n Price: $428.00
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HEK RNA: Total RNA prepared from Human Epidermal Keratinocytes, neonatal Size: 10 ug CAT.#: 102-R10n Price: $350.00
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HEK RNA, Neonatal: Total RNA prepared from Human Epidermal Keratinocytes, neonatal Size: 25 ug CAT.#: 102-R25n Price: $700.00
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Human EpiVita Basal Medium: Basal medium (contains no growth supplement).  Add GS before use. Size: 500 ml CAT.#: 140-500 Price: $74.00
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Human EpiVita Defined Growth Medium: All-in-one ready-to-use Size: 500 CAT.#: 141DGM-500 Price: $105.00
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Human EpiVita Defined Growth Medium Kit: Basal medium & growth supplement sold together packaged separately Size: Yields 500 ml CAT.#: 141DGMK-500 Price: $116.00
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Human EpiVita Defined Growth Supplement: Added to Basal Medium to create Growth Medium Size: 5 ml CAT.#: 141DGM-GS Price: $47.00
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Human EpiVita Serum-Free Growth Medium for Adult Cells: All-in-one ready-to-use, standard Epithelial Cell Medium, for adult cells Size: 500 CAT.#: 141-500a Price: $105.00
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Human EpiVita Serum-Free Growth Medium for Neonatal Cells: All-in-one ready-to-use, standard Epithelial Cell Medium, for neonatal and fetal cells Size: 500 CAT.#: 141-500 Price: $103.00
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Human EpiVita Serum-Free Growth Medium Kit for Adult Cells: Basal medium & growth supplement sold together packaged separately, for adult cells Size: Yields 500 ml CAT.#: 141K-500a Price: $116.00
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Human EpiVita Serum-Free Growth Medium Kit for Neonatal Cells: Basal medium & growth supplement sold together packaged separately, for neonatal and fetal cells Size: Yields 500 ml CAT.#: 141K-500 Price: $114.00
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Human EpiVita Serum-Free Growth Supplement for Adult Cells: Added to Basal Medium to create Growth Medium, for adult cells Size: 5 ml CAT.#: 141-GSa Price: $47.00
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Human EpiVita Serum-Free Growth Supplement for Neonatal Cells: Added to Basal Medium to create Growth Medium, for neonatal and fetal cells Size: 5 ml CAT.#: 141-GS Price: $45.00
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Extended Family Products

Product Size CAT.# Price Quantity
Cyto-X Cell Counting Reagent: 500 tests Size: 1 Bottle CAT.#: 028-01 Price: $139.00
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Cyto-X Cell Counting Reagent Sample: 100 tests Size: Sample CAT.#: 028-S Price: $36.00
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HBSS: 1X HBSS for washing of cells before Trypsin/EDTA treatment Size: 100 ml CAT.#: 062-100 Price: $18.00
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Trypsin Neutralization Solution: Stops the trypsinization reaction. Size: 100 ml CAT.#: 080-100 Price: $18.00
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Trypsin/EDTA Solution: For subculturing of adherent cells. Size: 100 ml CAT.#: 070-100 Price: $18.00
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