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BAOEC: Bovine Aortic Endothelial Cells



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A:Bovine Aortic Endothelial Cells, BAOEC. B&C: BAOEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit.

Bovine Aortic Endothelial Cells (BAOEC) are derived from the aorta of USDA-inspected cattle. They are cryopreserved at second passage, and can be cultured and propagated at least 16 population doublings. These cells play critical roles in cardiac homeostasis. Changes in structure and function of endothelial cells have been linked to vascular diseases such as thrombosis1,2, atherosclerosis3, and hypertension4. They are an economical alternative suitable for the studies of endothelial function and endothelial metabolism. A more sophisticated cell culture system, closer to the in vitro interactions of endothelia and smooth muscle cells, is generated by co-culture with species-matched bovine arterial smooth muscle cells.

  1. Jaffe, E.A., New Engl. J. Med. 296:377 (1977).
  2. Mason, R.G. et al, Arch. Pathol. Lab. Med. 101:61 (1977).
  3. Ross, R.N., Nature 362: 801 (1993).
  4. Ryan, U.S., Handbook of Physiology: The Respiratory System,  p 351 (1985).
Products are for research use only. They are not intended for human, animal, or diagnostic application.
Animal tissues were USDA inspected. 

Tissue:
Aorta of USDA-inspected cattle
Cryopreserved ampoule:
2nd passage, >500,000 cells in Bovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved BAOEC(B304-05), 500 ml Bovine Endothelial Cell Growth Medium (B211-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Bovine Endothelial Cell Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Cytofect Endothelial Cell Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved BAOEC Each kit contains an ampoule of >500,000 cryopreserved cells, 500 ml Bovine Endothelial Cell Growth Medium, and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		B304K-05
B304-05 		$534
$395
Proliferating BAOEC Cultured and shipped in Bovine Endothelial Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		B305-25
B305-75
B305-6w
B305-96w 		$395
$585
$585
$705
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Bovine EC Growth Medium Optimized and ready for use 500 ml B211-500 $98
Bovine EC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml B211K-500 $105
Bovine EC Basal Medium Add Growth Supplement before use 500 ml B210-500 $53
Bovine EC Supplement Growth Added to Basal Medium to create Growth Medium 50 ml B211-GS $47
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
BAOEC RNA Total RNA prepared from Bovine Aortic Endothelial Cells 10 ug
25 ug 		B304-R10
B304-R25 		$295
$590