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Human Umbilical Vein Smooth Muscle Cells: HUVSMC



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Human Umbilical Vein Smooth Muscle Cells, HUVSMC

Human umbilical vein smooth muscle cells (HUVSMC) are derived from the umbilical vein. They are cryopreserved at second passage. HUVSMC can be cultured and propagated 6 population doublings. Human umbilical vein actively regulates its vascular tone under the control of pO2. Vasodilation endothelial prostanoids contribute to the hypoxic vasodilation that counteracts the vasoconstricting actions of prostanoids released from HUVSMC. It was found that vasoconstricting prostanoids are released from the endothelium of the human umbilical vein at hyperoxia1. During preeclampsia a total loss of neuronal nitric oxide synthase (nNOS) protein expression and a significant decrease in mRNA expression were seen. It was found that such loss might alter the regulation of blood flow in the fetal and maternal placental vasculature2. Both ouabain and marinobufagenin activate proliferation of the HUVSMC. The concentration ranges of both compounds were found to be below that necessary to induce cytoplasmic ion alterations by sodium pump inhibition3. 
  1.  Mildenberger E, et al. J. Perinat. Med. 2004; 32(2):149-54.
  2.  Schonfelder G, et al. Histopathology. 2004 Feb; 44(2): 116-28.
  3.  Abramowitz J, et al. Circulation. 2003 Dec 16; 108(24): 3048-53.
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human normal umbilical vein
Cryopreserved ampoule:
2nd passage, >500,000 cells in Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HUVSMC (250-05n), 500 ml Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Smooth Muscle Cell Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 6 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HUVSMC, neonatal Each kit contains an ampoule of >500,000 cryopreserved cells (250-05n), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		250K-05n
250-05n 		$634
$495
Proliferating HUVSMC, neonatal Cultured and shipped at third passage in Smooth Muscle Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		251-25n
251-75n
251-6Wn
251-96Wn 		$495
$685
$685
$805
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Smooth Muscle Cell Growth Medium Optimized and ready for use . 500 ml 311-500 $98
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500 $105
Smooth Muscle Cell Basal Medium Add Growth Supplement before use. 500 ml 310-500 $53
Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 311-GS $53
Smooth Muscle Cell Differentiation    250 ml 311D-250 $68
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51