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Human Umbilical Artery Endothelial Cells: HUAEC



(Enlarge Image)

Right: Human Umbilical Artery Endothelial Cells, HUAEC
Left: HUAEC immunofluorescently stained with anti-vWF antibody and nucleus stained with PI.

Human Umbilical Artery Endothelial Cells (HUAEC) are isolated from normal human umbilical arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. Arterial endothelial cells have been used as an in vitro model for the study of morphological and ultrastructural changes in the development of atherosclerosis1. The HUAEC system has also been used for the study of endothelial cell-dependent lymphocyte activation .and chemoattractant activity2,3.
  1. Antonor, A.S. et al, Atherosclerosis 59:1 (1986).
  2. Ashida, E.R. et al, J. Clin. Inv. 67:1490 (1981).
  3. Farber, H.W. et al, Amer. J. Physiol. 262 (4 parts):H1088 (1992).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human normal umbilical arteries
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HUAEC(202-05n), 500 ml MesoEndo Endothelial Cell Growth Medium (212-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 15 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HUAEC, neonatal Each kit contains an ampoule of >500,000 cryopreserved cells (202-05n), 500 ml of MesoEndo Endothelial Cell Growth Medium (212-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		202K-05n
202-05n 		$526
$383
Proliferating HUAEC, neonatal Cultured at second passage in MesoEndo Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		203-25n
203-75n
203-6Wn
203-96Wn 		$383
$573
$573
$693
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
MesoEndothelial Cell Growth Medium Optimized and ready for use. 500 ml 212-500 $102
MesoEndothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 212K-500 $110
Endothelial Basal Medium Add Growth Supplement before use 500 ml 210-500 $53
MesoEndothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 212-GS $58
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HUAEC RNA, neonatal Total RNA prepared from HUAEC 10 ug
25 ug 		202-R10n
202-R25n 		$325
$650