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Human Umbilical Vein Endothelial Cells: HUVEC



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A: Human Umbilical Vein Endothelial Cells, HUVEC. B: HUVEC immunofluorescently stained with anti-VEGFR2 (Cat# CC1020) and nucleus stained with PI. C&D: HUVEC transfected with GFP plasmid DNA using the Cytofect™ HUVEC Transfection Kit.

Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. HUVEC are responsive to cytokine stimulation in the expression of cell adhesion molecules. These cell systems are commonly used for physiological and pharmacological investigations, such as macromolecule transport1, blood coagulation2, and fibrinolysis3.
 
1. Weiss, T.L. et al, In Vitro Cell. Biol. 26:759 (1990).
2. Furie, B. et al, Cell 53:505 (1988).
3. Wojta, J. et al, Blood 81(12):3285 (1993).
 
 
 
Endothelial Cells play a pivotal role in a diverse range of physiological processes, such as angiogenesis and as the selective blood barrier; and pathophysiological processes, including arterial disease and cancer development. At Cell Applications, select lots of HUVEC, HAOEC, & HMVEC undergo an additional set of characterization to demonstrate stiumlation-dependent angiogenesis and other endothelial cell signaling pathways. 
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human normal umbilical vein
Cryopreserved ampoule:
Primary culture, >500,000 cells in Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HUVEC (200-05n), 500 ml Endothelial Cell Growth Medium (211-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Endothelial Cell Transfer Medium at 1st passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Cytofect HUVEC Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HUVEC, neonatal Each kit contains an ampoule of >500,000 cryopreserved cells (200-05n), 500 ml of Endothelial Cell Growth Medium (211-500), a Subculture Reagent Kit. 1 Kit
1 Ampoule 		200K-05n
200-05n 		$389
$250
Proliferating HUVEC, neonatal Cultured at first passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		201-25n
201-75n
201-6Wn
201-96Wn 		$250
$440
$440
$560
Cryopreserved HUVEC, pooled Each kit contains an ampoule of >500,000 cryopreserved cells (200p-05n), 500 ml of Endothelial Cell Growth Medium (211-500), a Subculture Reagent Kit. 1 Kit
1 Ampoule 		200pK-05n
200p-05n 		$324
$185
Proliferating HUVEC, pooled Cultured at first passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		201p-25n
201p-75n
201p-6Wn
201p-96Wn 		$185
$375
$375
$495
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Endothelial Cell Growth Medium Optimized and ready for use 500 ml 211-500 $98
Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 211K-500 $105
Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 210-500 $53
Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 15 ml 211-GS $53
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HUVEC RNA , neonatal Total RNA prepared from HUVEC 10 ug
25 ug 		200-R10n
200-R25n 		$325
$650
Stimulated HUVEC RNA , neonatal Total RNA prepared from stimulated HUVEC 10 ug
25 ug 		200S-R10n
200S-R25n 		$380
$760