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Human Marrow Stromal Cells: HMSC



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Human Marrow Stromal Cells, HMSC

Human Marrow Stromal Cells (HMSC) are isolated from human bone marrow. They are cryopreserved at second passage and can be cultured and propagated 10 population doublings.  In addition to supporting hematopoietic cells1, marrow stromal cells can be induced to differentiate into cells of different connective tissue lineage, such as bone, cartilage, and fat2,3,4. The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue5,6. Recent reports indicate that HMSCs are capable of cell fates crossing germ layer boundaries. In addition to differentiating into multi-lineages of the mesoderm, these cells can also differentiate into neurons7 of ectodermal origin and hepatocyte-like cells8 of endodermal origin.

  1. Verfaillie, C.M., Blood 82:1045 (1993).
  2. Bruder, S.P. et al, J. Cell. Biochem. 64:278 (1997).
  3. Mackay, A..M. et al, Tissue Engineering 4:415 (1998).
  4. Pittenger, M.F. et al, Science 284:143 (1999).
  5. Horwitz, E.M. et al, Blood 92 Suppl 1:249 (1998).
  6. Bruder, S.P. et al, J. Orthop. Res. 16:155 (1998).
  7. Woodbury, D., et al, J. Neurosci. Res. 61:364 (2000).
  8. Oh, S.H., et al, Biochem. Biophy. Res. Commun. 279:500 (2000).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human bone marrow
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HMSC (492-05f), 500 ml Human Marrow Stromal Cell Growth Medium (419-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
 Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HMSC, fetal Each kit contains an ampoule of >500,000 cryopreserved cells (492-05f), 500 ml of Human Marrow Stromal Cell Growth Medium (419-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		492K-05f
492-05f 		$789
$635
Proliferating HMSC, fetal Cultured at second passage in Human Marrow Stromal Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		493-25f
493-75f
493-6wf
493-96wf 		$635
$825
$825
$945
Cryopreserved HMSC, adult Each kit contains an ampoule of >500,000 cryopreserved cells (492-05a), 500 ml of Human Marrow Stromal Cell Growth Medium (419-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		492K-05a
492-05a 		$809
$655
Proliferating HMSC, adult Cultured at second passage in Human Marrow Stromal Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		493-25a
493-75a
493-6Wa
493-96Wa 		$655
$845
$845
$965
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Human Marrow Stromal Cell Growth Medium Optimized and ready for use. 500 ml 419-500 $113
Human Marrow Stromal Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500ml 419K-500 $121
Human Marrow Stromal Cell Basal Medium Add Growth Supplements before use. 500 ml 418-500 $58
Human Marrow Stromal Cell Growth Supplements Added to Basal Medium to create Growth Medium 30 ml 419-GS $63
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51