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Human Epidermal Melanocytes: HEM



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Human Epidermal Melanocytes, HEM

Human Epidermal Melanocytes (HEM) are primary cells isolated from normal human neonatal foreskin. HEM are cryopreserved at second passage and can be cultured and propagated at least 12 population doublings. These epidermal melanocytes produce melanin, thus a useful cell model in the study of hyperpigmentation through accentuated melanocyte proliferation and differentiation1,2, as well as progression of melanocytic neoplasia3,4.
  1. Jimbow, K. et al, J. Invest. Dermatol. 78:108 (1982).
  2. lmokawa, G. et al, Arch. Dermatol. Res. 278:352 (1986).
  3. Herlyn, M. et al, Lab. Invest. 56:461(1987)
  4. Simon, H., Cancer Res. 56:3112 (1996).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human normal neonatal foreskin
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 30% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HEM (104-05n), 500 ml Melanocyte Growth Medium (135-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HEM, neonatal Each kit contains one ampoule of >500,000 cells, Melanocyte Growth Medium (135-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		104K-05n
104-05n 		$583
$445
Proliferating HEM, neonatal Cultured and shipped at third passage in Melanocyte Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		105-25n
105-75n
105-6Wn
105-96Wn 		$445
$635
$635
$755
Cryopreserved HEM, adult Each kit contains one ampoule of >500,000 cells, Melanocyte Growth Medium (135-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		104K-05a
104-05a 		$613
$475
Proliferating HEM, adult Cultured and shipped at third passage in Melanocyte Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		105-25a
105-75a
105-6Wa
105-96Wa 		$475
$665
$665
$785
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Melanocyte Growth Medium Optimized and ready for use. 500 ml 135-500 $97
Melanocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 135K-500 $105
Melanocyte Basal Medium Add Growth Supplement before use 500 ml 134-500 $53
Melanocyte Growth Supplement Added to Basal Medium to create Growth Medium 5 ml (3 parts) 135-GS $53
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
Melanocyte RNA , neonatal Total RNA prepared from HEM 10 ug
25 ug 		104-R10n
104-R25n 		$325
$650