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Human Epidermal Keratinocytes: HEK



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A: Human Epidermal Keratinocytes, HEK. B: HEK stained for cytokeratin peptide 14. C&D: HEK transfected with GFP plasmid DNA using the Cytofect™ Epithelial Cell Transfection Kit.

Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. HEK is a suitable in vitro model for dermal toxicology tests in screening new skin products1, and for developing dermal skin substitutes2. HEK has been used in numerous applications, including investigations of human epidermal development3, differentiation4, and cellular aspects of skin diseases5.
  1. Delco, V. et al, Alternative Methods in Toxicology, 3: 466 (1985).
  2. Joyce, S.T. et al, J. Biomed. Mater. Res. 22:937 (1988).
  3. Holbrook, K.A., Biochemistry and Physiology of the Skin, Vol. 1:64 (1983).
  4. Fuchs, F.J., Cell Biol. 111 (No. 6, Pt. 2):2807 (1990).
  5. Koizami, H. et al, British J. Dermatol. 134: 686 (1996).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human neonatal foreskin or adult skin
Cryopreserved ampoule:
Primary culture, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HEK (102-05), 500 ml HEK Growth Medium (131-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Keratinocyte Serum-Free Growth Medium at 1stpassage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Cytofect Epithelial Cell Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HEK, adult Each kit contains an ampoule of cryopreserved HEK (>500,000 cells in an ampoule), 500 ml of HEK Cell Growth Medium (131-500) and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		102K-05a
102-05a 		$493
$365
Proliferating HEK, adult Cultured and shipped at first passage in Keratinocyte Serum-Free Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		103-25a
103-75a
103-6Wa
103-96Wa 		$365
$555
$555
$678
Cryopreserved HEK, fetal Each kit contains an ampoule of cryopreserved HEK (>500,000 cells in an ampoule), 500 ml of HEK Cell Growth Medium (131-500) and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		102K-05f
102-05f 		$603
$475
Proliferating HEK, fetal Cultured and shipped at first passage in Keratinocyte Serum-Free Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		103-25f
103-75f
103-6Wf
103-96Wf 		$475
$665
$665
$785
Cryopreserved HEK, neonatal Each kit contains an ampoule of cryopreserved HEK (>500,000 cells in an ampoule), 500 ml of HEK Cell Growth Medium (131-500) and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		102K-05n
102-05n 		$508
$380
Proliferating HEK, neonatal Cultured and shipped at first passage in Keratinocyte Serum-Free Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		103-25n
103-75n
103-6Wn
103-96Wn 		$380
$570
$570
$690
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
HEK Growth Medium for fetal and neonatal cells Optimized and ready for use 500 ml 131-500 $87
HEK Growth Medium Kit for fetal and neonatal cells Basal Medium & Growth Supplement packaged separately. Yields 500 ml 131K-500 $95
HEK Basal Medium for fetal and neonatal cells Add Growth Supplement before use 500 ml 130-500 $53
HEK Growth Supplement for fetal and neonatal cells Added to Basal Medium to create Growth Medium 5 ml (3 parts) 131-GS $42
HEK Growth Medium for adult cells Optimized and ready for use 500 ml 131-500a $97
HEK Growth Medium Kit for adult cells Basal Medium & Growth Supplement packaged separately. Yields 500 ml 131K-500a $105
HEK Basal Medium for adult cells Add Growth Supplement before use 500 ml 130-500a $58
HEK Growth Supplement for adult cells Added to Basal Medium to create Growth Medium 5 ml (3 parts) 131-GSa $47
HEK Calcium-Free Growth Medium All-in-one ready-to-use media, without Calcium 500 ml 133-500 $95
HEK Calcium-Free Growth Medium Kit Basal Medium and Growth Supplement sold together, bottled separately, without Calcium Yields 500 ml 133K-500 $100
HEK Calcium-Free Basal Medium Add Growth Supplement before use 500 ml 132-500 $58
HEK Calcium-Free Growth Supplement Added to Basal Medium to create Growth Medium 5 ml (2 parts) 133-GS $42
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HEK RNA, adult Total RNA prepared from HEK 10 ug
25 ug 		102-R10a
102-R25a 		$325
$650
HEK RNA, neonatal Total RNA prepared from HEK 10 ug
25 ug 		102-R10n
102-R25n 		$325
$650