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A: Human Dermal Fibroblasts, HDF. B: HDF immunofluorescently stained with anti-FSP antibody. C&D: HDF transfected with GFP plasmid DNA using CytofectTM Fibroblast Transfection Kit. |
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Human Dermal Fibroblasts (HDF) are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings. Fibroblasts are found in all connective tissues, and they synthesize and secrete extracellular matrix proteins under cell culture conditions1. They are a well established system for in vitro analysis of fibroblast growth, migration and collagen metabolism2-5 in wound healing. Fibroblasts grown in a biodegradable mesh have been used as a living dermal replacement6,7.
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Hedman, K. et al, J. Cell Biol. 81:83 (1979).
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Gay, S. et al, Proc. Natl. Acad. Sci. 73:4037 (1976).
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Booth, A.B. et al, Biochem. Biophys. Acta 607:145 (1980).
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Peterkofsky, B. et al, Proc. Natl. Acad. Sci. 53:335 (1965).
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Hausmann, E., Biochem. Biophys. Acta 133:591 (1967).
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Hansborough, J.F. et al, Surgery 111(4):438 (1991).
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Cooper, M.L. et al, Biomaterials 12:243 (1991).
Characterization: Positive for fibroblast surface protein.
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
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Tissue:
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Normal human neonatal foreskin or adult skin
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Cryopreserved ampoule:
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Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
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Kit contains:
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Ampoule of cryopreserved HDF (106-05), 500 ml Growth Medium (116-500), and a Subculture Reagent Kit (090K).
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Proliferating Cells:
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Shipped in Growth Medium at 1st passage in either flasks or multiwell dishes.
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Population doublings:
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Can be cultured at least 16 doublings
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