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Human Placental Epithelial Cells: HPlEpC



(Enlarge Image)

Left: Human Placental Epithelial Cells, HPlEpC
Right: HPlEpC immunofluorescently stained with anti-CK18 antibody

Human Placental Epithelial Cells (HPlEpC) are isolated from normal human amnion. They are cryopreserved at the end of primary culture. These epithelial cells proliferate well when they are thawed and plated from cryopreservation, but they do not plate and proliferate after trypsinization. HPlEpC has been used to study the secretion of activin A1, the neural formation during early fetal development2, and the antimicrobial and endotoxin-neutralizing barrier effect of Histones H2A and H2B against microorganism3. Prostaglandin E2 induces high level of amniotic epithelial cell death via necrosis rather than apoptosis4. Matrix metalloproteinases–9 (MMP-9) localized mainly in amnion epithelia. An increase in MMP-9 expression may contribute to degradation of extracellular matrix in fetal membrane and placenta, thereby facilitate fetal membrane rupture and placental detachment5.
  1. Schneider-Kolsky, M.E., et al, Placenta 23(4):294-302 (2002).
  2. Koyano, S. et al, Dev. Growth Differ.44(2):103-12 (2002).
  3. Kim, H.S, et al, J. Immunol. 168(5):2356-64 (2002).
  4. Surendran, S. Inflamm. Res. 50(10):483-5 (2001).
  5. Xu, P. et al, J. Clin. Endocrinol. Metab. 87(3):1353-61 (2002).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human amnion
Cryopreserved ampoule:
>500,000 of primary culture cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HPlEpC (230-05), 500 ml Epi Growth Medium (215-500), and a Subculture Reagent Kit (090K).
Proliferating Cells: 
Shipped in Growth Medium in either flasks or multiwell dishes.
Population doublings:
n/a

 
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HPlEpC, fetal Each kit contains an ampoule of cryopreserved HPlEpC (230-05f), 500 ml of Epi Growth Medium (215-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		230K-05f
230-05f 		$721
$565
Proliferating HPlEpC, fetal Cultured at first passage in Epi Growth Medium T-25 Flask
T-75 Flask
6 well
96 well 		231-25f
231-75f
231-6Wf
231-96Wf 		$565
$755
$755
$875
Cryopreserved HPlEpC, neonatal Each kit contains an ampoule of cryopreserved HPlEpC (230-05n), 500 ml of Epi Growth Medium (215-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		230K-05n
230-05n 		$721
$565
Proliferating HPlEpC, neonatal Cultured at first passage in Epi Growth Medium T-25 Flask
T-75 Flask
6 well
96 well 		231-25n
231-75n
231-6Wn
231-96Wn 		$565
$755
$755
$875
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Epi Growth Medium Optimized and ready for use 500 ml 215-500 $115
Epi Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 215K-500 $125
Epi Basal Medium Add Growth Supplement before use 500 ml 214-500 $68
Epi Growth Supplement Added to Basal Medium to create Growth Medium 5 ml (3 parts) 215-GS $57
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HPlEpC RNA, fetal Total RNA prepared from HPlEpC 10 ug
25 ug 		230-R10f
230-R25f 		$325
$650
HPlEpC RNA, neonatal Total RNA prepared from HPlEpC 10 ug
25 ug 		230-R10n
230-R25n 		$325
$650