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Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC



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Left: Cell Applications Human Dermal Microvascular Endothelial Cells, CADMEC/HMVEC. 
Right: CADMEC immunofluorescently stained with anti-CD31 antibody (Cat# CB13678) and nucleus counterstained with PI.

 

Cell Applications' Dermal Microvascular Endothelial Cells (CADMEC™/HMVEC) are isolated from normal human neonatal foreskin or adult skin capillaries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. They are responsive to cytokine stimulation in the expression of cell adhesion molecules. CADMEC™ express CD36 constitutively, while large vessel endothelial cells do not. CADMEC™ is an organ-specific endothelial cell system for the study of angiogenesis1, tumor metastasis2,3, wound healing4, inflammation5,6, macromolecule transport7, and drug metabolism8. There is growing evidence of differences in physiology and pharmacology between microvascular endothelial cells and macrovascular endothelial cells9,10.
 
Pre-Screened HMVEC/CADMEC: Cell Applications, Inc. provides special lots that are verified as responsive to VEGF. The activation of VEGF-R2 was validated by assessing autophosphorylation at tyrosine 1175. VEGF-R2 is a high affinity receptor for VEGF which stimulates proliferation, chemotaxis and sprouting of endothelial cells in vitro and angiogenesis in vivo via VEGF-R2 signaling pathway. More information about these endothelial cells can be found on the Pre-Screened Endothelial Cell Product Page.
 
Characterization:
  • Positive for factor VIII-related antigen expression.
  • Positive for CD36 expression.
  • Positive for metabolic activity by DiI-Ac-LDL uptake.
  • Positive for functional activity by cytokine-stimulated  leukocyte adherence.
References:
1. Swerlick, R.A. et.al, J. Invest. Dermatol. 97(2):190 (1991)
2. Folkman, J. et al, Nature 288:551 (1980).
3. Lee, K.H. et al, J. Invest. Dermatol. 98(1):79 (1992).
4. Deryugina, E.I. et al, Hybridoma 15(4):279 (1996)
5. Sepp, N.T. et al, J. Invest. Dermatol. 104(2):277 (1995).
6. Swerlick, R.A. et al, J. Invest. Dermatol. 100(1):111S (1993).
7. Hanasaki, K. et al, J. Bio. Chem. 270(13):7533 (1995)
8. Pruckler, J.M. et al, Pathobiology 61:283 (1993).
9. Foster, C.A. et al, J. Dermatol. 21(11):847 (1994).
10. Schnitzer, J.E. et al, BBRC. 199(1):11 (1994).
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
 

Tissue:
Normal human neonatal foreskin or adult skin capillaries
Cryopreserved ampoule:
2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Each kit contains an ampoule of cryopreserved CADMEC (100-05), 500 ml of CADMEC Cell Growth Medium (112-500), a Subculture Reagent Kit, two Extracellular Matrix Attachment Factor Coated T-25 Flasks (121-25-02), 100 ml of Attachment Factor Solution (123-100).
Proliferating Cells:
 
Shipped in Endothelial Cell Transfer Media at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved CADMEC, adult Each kit contains an ampoule of cryopreserved CADMEC (100-05), 500 ml of CADMEC Cell Growth Medium (112-500), a Subculture Reagent Kit, two Extracellular Matrix Attachment Factor Coated T-25 Flasks (121-25-02), 100 ml of Attachment Factor Solution (123-100). 1 Kit
1 Ampoule 		100K-05a
100-05a 		$732
$540
Proliferating CADMEC, adult Cultured at third passage in CADMEC Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		101-25a
101-75a
101-6Wa
101-96Wa 		$540
$730
$730
$850
Cryopreserved CADMEC, neonatal Each kit contains an ampoule of cryopreserved CADMEC (100-05), 500 ml of CADMEC Cell Growth Medium (112-500), a Subculture Reagent Kit, two Extracellular Matrix Attachment Factor Coated T-25 Flasks (121-25-02), 100 ml of Attachment Factor Solution (123-100). 1 Kit
1 Ampoule 		100K-05n
100-05n 		$712
$520
Proliferating CADMEC, neonatal Cultured at third passage in CADMEC Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		101-25n
101-75n
101-6Wn
101-96Wn 		$520
$710
$710
$830
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
CADMEC Growth Medium Optimized and ready for use 500 ml 112-500 $105
CADMEC Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 112K-500 $116
CADMEC Basal Medium Add Growth Supplement before use 500 ml 110-500 $53
CADMEC Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 112-GS $63
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
Cord Formation Medium - CADMEC Serum-Free, Growth Factor-Free Medium A proprietary medium developed to induce morphological differentiation of CADMEC in Attachment Factor Solution coated tissue culture ware in 50% confluent culture. This medium induces CADMEC to form cord-like structures in 48 hours. These differentiated cells will resume their cobblestone morphology when switched back to CADMEC Growth Medium. It is supplemented with defined components to allow angiogenesis studies on the Attachment Factor-coated tissue culture ware without the interference of undefined components in Matrigel. Available without Phenol Red (114PR-500). 500 ml
500 ml 		114-500
114PR-500 		$105
$110
Endothelial Cell Starvation Medium Endothelial Cell Starvation Medium. Available without Phenol Red (209PR-250) 250 ml
250 ml 		209-250
209PR-250 		$58
$68
Attachment Factor Solution Promotes attachment of endothelial cells. 100 ml 123-100 $18
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
CADMEC RNA , adult Total RNA prepared from CADMEC 10 ug
25 ug 		100-R10a
100-R25a 		$325
$650
Stimulated CADMEC Total RNA, adult Total RNA prepared from stimulated CADMEC 10 ug
25 ug 		100S-R10a
100S-R25a 		$380
$760
CADMEC RNA, neonatal Total RNA prepared from CADMEC 10 ug
25 ug 		100-R10n
100-R25n 		$325
$650
Stimulated CADMEC Total RNA, neonatal Total RNA prepared from stimulated CADMEC 10 ug
25 ug 		100S-R10n
100S-25n 		$380
$760