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Human Mammary Epithelial Cells: HMEpC



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A: Human Mammary Epithelial Cells,HMEpC B&C: HMEpC transfected with GFP plasmid DNA using the Cytofect™ Epithelial Cell Transfection Kit.

Human Mammary Epithelial Cells (HMEpC) are derived from normal adult mammary glands. They are cryopreserved at fifth passage. HMEpC can be cultured and propagated 16 population doublings. It was shown that normal HMEpC have a high amount of cyclin-dependent kinase 6 activity, which restrains HMEpC proliferation, and loss or down-regulation of cdk 6 may play a role in breast tumor development1. Transforming HMEpC to immortality and malignancy was a useful model for studying early breast carcinogenesis2. Autocrine colony-stimulating factor receptors may contribute to invasive progression in epithelial cancer was demonstrated in a 3-dimensional culture of HMEpC3.
  1. Lucas, J.J. et al, Mol. Cancer Res. 2(2):105 (2004).
  2. Stampfer, M.R. et al, J. Mammary Gland Biol. Neoplasia 5(4):365 (2000).
  3. Wrobel, C.N. et al, J. Cell Bio.165(2):263 (2004).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
 

Tissue:
Normal human mammary glands.
Cryopreserved ampoule:
5th passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HMEpC(830-05a), 500 ml of Mammary Epithelial Cell Growth Medium (815-500), & a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 6th passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Cytofect Epithelial Cell Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HMEpC, adult Each kit contains an ampoule of >500,000 cryopreserved cells (830-05a), 500 ml of Mammary Epithelial Cell Growth Medium (815-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		830K-05a
830-05a 		$728
$585
Proliferating HMEpC, adult Cultured and shipped at sixth passage in Mammary Epithelial Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		831-25a
831-75a
831-6Wa
831-96Wa 		$585
$775
$775
$885
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Mammary Epithelial Cell Growth Medium Optimized and ready for use 500 ml 815-500 $102
Mammary Epithelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 815K-500 $110
Mammary Epithelial Cell Basal Medium Add Growth Supplement before use 500 ml 814-500 $58
Mammary Epithelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 5 ml 815-GS $53
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HMEpC RNA, adult Total RNA prepared from HMEpC 10 ug
25 ug 		830-R10a
830-R25a 		$325
$650