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Human Lung Fibroblasts: HLF



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A: Human Lung Fibroblasts (HLF). B: HLF immunofluorescently stained with anti-FSP antibody. C&D: HLF transfected with GFP plasmid DNA using the Cytofect™ Fibroblast Transfection Kit.

Human Lung Fibroblasts (HLF) are derived from normal human lung parenchyma. They are cryopreserved at first passage and can be cultured and propagated at least 12 population doublings. Studies show HLF play a pivotal role in airway inflammation and it may be an important target for drugs used in asthma1. Fibroblast -based assay was used for screening of new antimicrobial drugs2. Human cytomegalovirus (HCMV) was found to completely down-regulate EGF receptor on the surface of fetal HLF3. Thrombin contributes to bronchoalveola lavage fluid mitogenicity in lung disease of the premature infant4. Pseudomonas autoinducer N-(3-oxododecanoyl) homoserine lactone induces cyclooxygenase-2 and prostaglandin E2 production in HLF important for inflammation and immune defenses5. Studies also show cigarette smoke extract added to the medium induces growth inhibition of HLF6.
  1. Oddera, S. et al, Int. Arch. Allergy Immunol. 129(2):152-9 (2002). 
  2. Takii, T. et al, Antimicrob. Agents Chemother. 46(8):2533-9 (2002).
  3. Beutler, T. et al, Am. J. Respir. Cell Mol. Biol. 28(1):86-94 (2003).
  4. Dik, W.A. et al, Pediatr. Pulmonol. 35(1):34-41 (2003).
  5. Smith, R.S. et al, J. Immunol. 169(5):2636-42 (2002).
  6. Nobukuni, S. et al, Respirology 7(3):217-23 (2002).

Characterization: Positive for fibroblast surface protein.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human lung parenchyma
Cryopreserved ampoule:
1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HLF (506-05a or 506-05f), 500 ml of HLF Growth Medium (516-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 12 doublings

Cytofect Fibroblast Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HLF, adult Each ampoule contains >500,000 cryopreserved cells. Each kit contains one ampoule, 500 ml Growth Medium (516-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		506K-05a
506-05a 		$653
$520
Cryopreserved HLF, fetal Each ampoule contains >500,000 cryopreserved cells. Each kit contains one ampoule, 500 ml Growth Medium (516-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		506K-05f
506-05f 		$653
$520
Proliferating HLF, adult Cultured and shipped at second passage in HLF Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		507-25a
507-75a
507-6Wa
507-96Wa 		$520
$710
$710
$830
Proliferating HLF, fetal Cultured and shipped at second passage in HLF Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		507-25f
507-75f
507-6Wf
507-96Wf 		$520
$710
$710
$830
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
HLF Growth Medium Optimized and ready for use 500 ml 516-500 $92
HLF Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 516K-500 $100
HLF Basal Medium Add Growth Supplement before use 500 ml 515-500 $53
HLF Growth Supplement Added to Basal Medium to create Growth Medium 50 ml 516-GS $47
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HLF RNA fetal Total RNA prepared from HLF 10 ug
25 ug 		506-R10f
506-R25f 		$325
$650