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Human Preadipocytes: HPAd



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Human Pre-Adipocytes, HPAd

Human Preadipocytes (HPAd) are derived from human adipose tissue. These fibroblast-like precursor cells are cryopreserved at the end of primary culture and can be propagated two passages prior to differentiating into Human Adipocytes (HAd). HPAd derived HAd were used to study insulin-stimulated glucose transport1,2, growth hormone enhanced lypolysis3 and obese gene expression4. HPAd can be fully differentiated into mature HAd in CAI’s Adipocyte Differentiation Medium. Significant differences in lipolysis and leptin production4 are observed in HPAd and HAd from human omental and subcutaneous adipose tissue. We provide HPAd from subcutaneous adipose tissue at various sites and adipose depot on the heart. HPAd/HAd are a useful in vitro cellular model for investigating the causes and treatment of obesity, type II diabetes and cardiovascular diseases. Recently it was discovered that human adipocytes might be a new target for the immunodeficiency virus-15.
  1. Tanti, J-F. et al., Endocrinology 138(5):2005 (1997)
  2. Okada, T. et al., J. Biochem Mol Biol 269(5):3568 (1994)
  3. Nam, S.Y. et al., Obes Rev 1(2):73 (2000)
  4. Hamilton, B.S. et al, Nature Medicine 1(9):953 (1995)
  5. van Harlemen, V. et al., Diabetes 51:2029 (2002)
  6. Hazan, U. et al., FASEB J. 16(10):1254 (2002) 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human adipose tissue
Cryopreserved ampoule:
2nd passage, >500,000 cells in Preadipocyte Cell Basal Medium that contains  20% FBS and 5% DMSO.
Kit contains:
Ampoule of cryopreserved HPAd (802h/s-05a), 500 ml of Preadipocyte Growth Medium (811-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured 2 passages before differentiation into HAd

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HPAd, adult, heart Each kit contains an ampoule of cryopreserved HPAd (802h-05a), 500 ml of Preadipocyte Growth Medium (811-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		802hK-05a
802h-05a 		$572
$439
Cryopreserved HPAd, adult, subcutaneous Each kit contains an ampoule of cryopreserved HPAd (802s-05a), 500 ml of Preadipocyte Growth Medium (811-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		802sK-05a
802s-05a 		$413
$280
Proliferating HPAd, adult, heart Cultured at third passage in Preadipocyte Growth Medium and shipped in Preadipocyte Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		803h-25a
803h-75a
803h-6Wa
803h-96Wa 		$439
$629
$629
$749
Proliferating HPAd, adult, subcutaneous Cultured at third passage in Preadipocyte Growth Medium and shipped in Preadipocyte Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		803s-25a
803s-75a
803s-6Wa
803s-96Wa 		$280
$470
$470
$590
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Preadipocyte Growth Medium Optimized & ready for use 500 ml 811-500 $92
Preadipocyte Basal Medium Add Growth Supplement before use 500 ml 810-500 $53
Preadipocyte Growth Supplement    50 ml 811-GS $47
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
Adipocyte Differentiation Medium Add to HPAd to differentiate into adipocytes 250 ml 811D-250 $68
Adipocyte Starvation Medium Starve adipocytes for 1 day prior to assays 250 ml 811S-250 $58
 
RNA
Product Name Product Description Size Cat. No. Price
HPAd RNA subcutaneous(s) Total RNA prepared from HPAd 10 ug
25 ug 		802s-R10a
802s-R25a 		$325
$650