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Human Adipocytes: HAd



(Enlarge Image)
Left: Human Adipocytes, HAd.
Right: Accumulated lipid droplets in HAd, stained by Oil Red O.

Previously, most research in adipocyte biology was conducted in mouse 3T3-L1 cell lines1,2,3. It was impossible to use human adipocytes because they die within 24 hours after isolation. To facilitate the study of human adipocyte biology, now we provide HAd (Human Adipocytes) which were differentiated from HPAd (Human Preadipocytes) through use of Adipocyte Differentiation Medium. Cells are shipped 10 to 14 days after initiating differentiation. Mature HAd are expected 15 days after induction of differentiation and should remain healthy and responsive for at least 2 weeks after complete differentiation to mature HAd. Adipose mass can be controlled by inhibition of HPAd differentiation and increase of lypolysis. HAd are ideal cellular models for drug discovery research in the area of obesity and diabetes.
  1. Russel, T. R. et al, Proc. Natl. Acad. Sci. USA 73:4516 (1976)
  2. Schmidt, W. et al, J. Biol. Chem. 265:15489 (1990)
  3. Spiegelman, B. M. et al, Cell 24:503 (1981)
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

 

 

Tissue:
Normal human adipose tissue
Cryopreserved ampoule:
n/a
Kit contains:
n/a
Proliferating Cells:
 
Shipped at first passage in Adipocyte Differentiation Medium, after 10 days of differentiation
Population doublings:
n/a

 
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Proliferating HAd, adult, subcutaneous(s) Cultured and shipped at first passage in Adipocyte Differentiation Medium. T-25 Flask
T-75 Flask
6 well
96 well 		803sD-25a
803sD-75a
803sD-6Wa
803sD-96Wa 		$175
$495
$495
$575
Proliferating HAd, adult, heart (h) Cultured and shipped at first passage in Adipocyte Differentiation Medium. T-25 Flask
T-75 Flask
6 well
96 well 		803hD-25a
803hD-75a
803hD-6Wa
803hD-96Wa 		$285
$635
$635
$715
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Adipocyte Differentiation Medium    250 ml 811D-250 $68
Adipocyte Differentiation Medium, component kit Differentiation Medium as individual components. Components 811DS-250 $85
Adipocyte Maintenance Medium    250 ml 811M-250 $63
Adipocyte Starvation Medium    250 ml 811S-250 $58
 
RNA
Product Name Product Description Size Cat. No. Price
HAd RNA, adult, subcutaneous Total RNA prepared from subcutaneously derived HAd. 10 ug
25 ug 		803sD-R10a
803sD-R25a 		$325
$650