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Human Subclavian Artery Endothelial Cells: HScAEC



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Right: Human Subclavian Artery Endothelial Cells, HScAEC
Left: HScAEC immunofluorescently stained with anti-CD31 antibody (Cat# CB13678) and nucleus stained with PI.

Human Subclavian Artery Endothelial Cells (HScAEC) are derived from normal human subclavian arteries. They are cryopreserved at second passage and can be cultured and propagated at least 10 population doublings. The shape and orientation of HScAEC was found to be strongly affected by blood flow conditions1. Autologous leukocyte adhesion to normal and pathologically altered subclavian arteries ex vivo was explored2. The effect of prolonged treatment with r-hirudin after angioplasty of the subclavian artery was evaluated in rabbits3, whereas rabbit ScAEC was used to study the effects of dietary and genetically induced hypercholesterolaemia on the vasodilator and antiaggregatory capacity of the endothelium 4.
  1. Sato, M. and Ohshima, N. Biorheology 31(2):143-53 (1994).
  2. Kennedy, S. et al, J. Immunol. Methods 244(1-2):153-62 (2000).
  3. Hadoke, P.W. et al, Coron. Artery Dis. 7(8):599-608 (1996).
  4. Greenlees, C. et al, Br. J. Pharmacol. 119(7):1470-6 (1996).
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human subclavian artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HScAEC (3010-05a), 500 ml of MesoEndo Endothelial Cell Growth Media (212-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HScAEC, adult Each kit contains an ampoule of cryopreserved HScAEC (3010-05a), 500 ml of MesoEndo Endothelial Growth Medium (212-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		3010K-05a
3010-05a 		$799
$656
Proliferating HScAEC, adult Cultured and shipped at third passage in MesoEndo Endothelial Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		3011-25a
3011-75a
3011-6Wa
3011-96Wa 		$656
$846
$846
$966
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
MesoEndo Endothelial Cell Growth Medium Optimized and ready for use 500 ml 212-500 $102
MesoEndo Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 212K-500 $110
Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 210-500 $53
MesoEndo Endothelial Cell Growth Supplements Added to Basal Medium to create Growth Medium 30 ml 212-GS $58
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HScAEC RNA, adult Total RNA prepared from HScAEC 10 ug
25 ug 		3010-R10a
3010-R25a 		$325
$650