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Human Aortic Smooth Muscle Cells: HAOSMC



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Human Aortic Smooth Muscle Cells, HAOSMC

Human Aortic Smooth Muscle Cells (HAOSMC) are derived from tunica intima and tunica media of normal human, fibrous plaque-free aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. Arterial smooth muscle cells are capable of synthesizing collagen, elastin, myosin and glycosaminoglycan1,2. Increased production of connective tissue components, hyperplasia and hypertrophy of intimal smooth muscle cells are found to gradually occlude the vessel lumen in atherosclerosis1,2. HAOSMC respond to various factors by proliferating or differentiating3,4. They are a well established cell system for the study of human vascular disorders such as atherosclerosis5,6 and stroke.

  1. Abraham, P.A. et al, Biochem. Biophys. Res. Comm. 58:597 (1974).
  2. Ross, R., New Engl. J. Med. 314(8):488 (1986).
  3. Fager, G. et al, In Vitro Cell. Biol. 25(6):511 (1989).
  4. Hoshi, H. et al, In Vitro Cell. Biol. 24(4):309 (1988).
  5. Orekhov, A.N. et al, Lab. Invest. 48:749 (1983).
  6. Jonasson, L. et al, Arteriosclerosis 6(2):131 (1986).
Characterization: Positive for smooth muscle cell specific a -actin expression.
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human tunica intima and tunica media of normal human, fibrous plaque-free aorta
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HAOEC(354-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Smooth Muscle Cell Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HAOSMC, adult Each kit contains an ampoule of cryopreserved > 500,000 cells, 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		354K-05a
354-05a 		$797
$659
Proliferating HAOSMC, adult Cultured and shipped at third passage in Smooth Muscle Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		355-25a
355-75a
355-6Wa
355-96Wa 		$659
$849
$849
$969
Cryopreserved HAOSMC, fetal Each kit contains an ampoule of cryopreserved > 500,000 cells, 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		354K-05f
354-05f 		$797
$659
Proliferating HAOSMC, fetal Cultured and shipped at third passage in Smooth Muscle Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		355-25f
355-75f
355-6Wf
355-96Wf 		$659
$849
$849
$969
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500 $98
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml (Kit) 311K-500 $105
Smooth Muscle Cell Basal Medium Add Growth Supplements before use 500 ml 310-500 $53
Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 311-GS $53
Smooth Muscle Cell Differentiation Medium    250 ml 311D-250 $68
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HAOSMC RNA, adult Total RNA prepared from HAOSMC 10 ug
25 ug 		354-R10a
354-R25a 		$325
$650