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Human Pulmonary Artery Smooth Muscle Cells: HPASMC



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Human Pulmonary Artery Smooth Muscle Cells, HPASMC

Human Pulmonary Artery Smooth Muscle Cells (HPASMC) are derived from tunica intima and tunica media of normal human, fibrous plaque-free pulmonary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. HPASMC are well suited for the study of pulmonary vascular disorders1, associated liver diseases2, and connective tissue diseases3. Phenotypic changes of smooth muscle cells have been found to be related to smooth muscle cell migration and intimal proliferation. Switches in smooth muscle cell phenotype lead to pulmonary hypertension and eventually heart and lung disorders4.
  1. Rabinovitch, M., Moss’ Heart Disease in Children, Infants and Adolescents, Williams and Wilkins, Baltimore, p. 856 (1989).
  2. Levine, O.R. et al, J. Pediatr. 83:964 (1973).
  3. Nair, S.S. et al, Arch. Intern. Med. 140:109 (1980).
  4. Rabinovitch, M., Toxicologic Pathology. Vol. 19, No. 4 (Pt.1) pp. 458-468 (1991).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human intrathoratic artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HPASMC (352-05a), 500 ml of Smooth Muscle Cell Media (311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HPASMC, adult Each kit contains an ampoule of cryopreserved HPASMC (352-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit. 1 Kit
1 Ampoule 		352K-05a
352-05a 		$758
$620
Proliferating HPASMC, adult Cultured and shipped at third passage in Smooth Muscle Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		353-25a
353-75a
353-6w
353-96w 		$620
$810
$810
$930
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500 $98
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 311K-500 $105
Smooth Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 310-500 $53
Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 311-GS $53
Smooth Muscle Cell Differentiation Medium    250 ml 311D-250 $68
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HPASMC RNA, adult Total RNA prepared from HPASMC 10 ug
25 ug 		352-R10a
352-R25a 		$325
$650