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Human Pulmonary Artery Endothelial Cells: HPAEC



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A: Human Pulmonary Artery Endothelial Cells, HPAEC B: HPAEC immunofluorescently stained with anti-CD31 antibody (Cat# CB13678) and nucleus stained with PI. C&D: HPAEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit.

Human Pulmonary Artery Endothelial Cells (HPAEC) are isolated from normal human pulmonary arteries. They are cryopreserved at second passage and can be cultured and propagated at least 15 population doublings. HPAEC possess an array of enzymatic activities1,2. They respond to a wide range of vasoactive substances3, commensurate with their control of blood pressure and blood pH in vivo . HPAEC have been used for the study of vascular permeability4 and inflammatory responses5. HPAEC in co-culture with HPASMC have been used as a model for pulmonary angiopathy6.

  1. Ryan, U.S. et al, Tissue Cell 8: 125-146 (1976).
  2. Ryan, U.S. et al, Clin. in Lab. Med. V. 3: 577 (1983).
  3. Ryan, U.S., Handbook of Physiology; The Respiratory System. Am. Physiol. Soc. Chap. 10.:351 (1985).
  4. Downie, G.H. et al, Am. J. Respir. Cell. Mol. Biol. 7:58 (1992).
  5. Farber, H.W. et al, Tissue & Cell 24 (3):355 (1992).
  6. Vender, R.L. et al, In Vitro Cell Dev. Biol. 28A:403 (1992).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

 

Tissue:
Normal human pulmonary artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HPAEC (302-05a), 500 ml of Endothelial Cell Media (211-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 15 doublings

Cytofect Endothelial Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HPAEC, adult Each kit contains an ampoule of >500,000 cells (302-05a), 500 ml of Growth Medium (211-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		302K-05a
302-05a 		$759
$620
Proliferating HPAEC, adult Cultured at third passage in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		303-25a
303-75a
303-6Wa
303-96Wa 		$620
$810
$810
$930
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Endothelial Cell Growth Medium Optimized and ready for use 500 ml 211-500 $98
Endothelial Cell Growth Medium Kitl Basal Medium & Growth Supplement packaged separately Yields 500 ml 211K-500 $105
Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 210-500 $53
Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 15 ml 211-GS $53
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HPAEC RNA, adult Total RNA prepared from HPAEC 10ug
25ug 		302-R10a
302-R25a 		$325
$650