Main Page >> >> Human Cardiac Fibroblasts: HCF
Human Cardiac Fibroblasts: HCF



(Enlarge Image)

A: Human Cardiac Fibroblasts, HCF. B: HCF immunofluorescently stained for anti-FSP antibody. C&D: HCF transfected with GFP plasmid DNA using the Cytofect™ Fibroblast Transfection Kit.

Human Cardiac Fibroblasts (HCF) are derived from normal human heart tissue. They are cryopreserved at first passage and can be cultured and propagated at least 8 population doublings. HCF has been used to study the effects of TNF-alpha and angiotensin (Ang) II levels on fibroblast function1. Expression of MyoD can induce myogenesis of cardiac fibroblasts2. Cardiac fibroblasts involved in aortic regurgitation synthesize more Fibronectin3. Estradiol inhibits cardiac fibroblast growth through conversion to methoxyestradiols, which may protect against remodeling involved in heart disease4. TGF-beta stimulates differentiation of fibroblasts to myofibroblasts, which increases collagen production5. Serum depletion of fibroblasts simulates one of the components of ischemia6.
  1. Peng, J. et al, Circ. Res. 91(12):1119-26 (2002).
  2. Etzion, S. et al, Circulation 106(12 Suppl. 1):I125-30 (2002).
  3. Borer, J.S. et al, Circulation 105(15):1837-42 (2002).
  4. Bubey, R.K. et al, Hypertension 39(2 pt 2):412-7 (2002).
  5. Petrov, V.V. et al, Hypertension 39(2):258-63 (2002).
  6. Leicht, M. et al, Cardiovasc. Res. 52(3):429-37 (2001).
Characterization: Positive for fibroblast surface protein.
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human heart tissue
Cryopreserved ampoule:
1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HCF (306-05f), 500 ml of Cardiac/Lung Fibroblast Growth Medium (516-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 8 doublings

Cytofect Fibroblast Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HCF, fetal Each kit contains one ampoule of >500,000 cells, 500 ml of Lung/Cardiac Fibroblast Growth Medium (516-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		306K-05f
306-05f 		$728
$595
Proliferating HCF, fetal Cultured and shipped at second passage in HCF Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		307-25f
307-75f
307-6Wf
307-96Wf 		$595
$785
$785
$905
Cryopreserved HCF, adult Each kit contains one ampoule of >500,000 cells, 500 ml of Lung/Cardiac Fibroblast Growth Medium (516-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		306K-05a
306-05a 		$728
$595
Proliferating HCF, adult Cultured and shipped at second passage in HCF Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		307-25a
307-75a
307-6Wa
307-96Wa 		$595
$785
$785
$905
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Lung/Cardiac Fibroblast Growth Medium Optimized and ready for use. 500 ml 516-500 $92
Lung/Cardiac Fibroblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 516K-500 $100
Lung/Cardiac Fibroblast Basal Medium Add Growth Supplement before use. 500 ml 515-500 $53
Lung/Cardiac Fibroblast Growth Supplement Added to Basal Medium to create Growth Medium. 50 ml 516-GS $47
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HCF RNA, fetal Total RNA prepared from HCF. 10 ug
25 ug 		306-R10f
306-R25f 		$325
$650