BACKGROUND Transfection, the introduction of DNA into cells, is a powerful technique used to study in vivo gene function and regulation. The Cytofect-Endothelial Cell Transfection kit is a plasmid DNA delivery system specifically optimized to deliver DNA into a wide variety of endothelial cells. This kit contains Cytofect-2, Enhancer, and Media for transfection and culturing cells. Peptide Enhancer complexes with DNA and Cytofect-2 and escorts the tranfection complex to the nucleus. The result has been high transfection efficiency and viability for these traditionally hard-to-transfect primary endothelial cells.
This Cytofect-Endothelial Cell Transfection Kit contains all the necessary reagents and media for transfection. Optimal transfection protocols are provided in the kit to carry out successful transfection of the following endothelial cells with high efficiency (40%-85%) and viability (80%-95%):
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HUVEC: Human Umbilical Vein Endothelial Cells
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HAOEC: Human Aortic Endothelial Cells
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HCAEC: Human Coronary Artery Endothelial Cells
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HPAEC: Human Pulmonary Artery Endothelial Cells
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HLMVEC: Human Lung Microvascular Endothelial Cells
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BAOEC: Bovine Aortic Endothelial Cells
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BCAEC: Bovine Coronary Artery Endothelial Cells
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BPAEC: Bovine Pulmonary Artery Endothelial Cells
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RAOEC: Rat Aortic Endothelial Cells
Products are for research use only. They are not intended for human, animal, or diagnostic applications. TR1 and PE are manufactured by Targeting Systems.
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