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Human Carotid Artery Endothelial Cells: HCtAEC



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Left: Human Carotid Artery Endothelial Cells, HCtAEC.
Right: HCtAEC immunofluorescently stained with anti-vWF antibody and nucleus counterstained with PI.

 

Human Carotid Artery Endothelial Cells (HCtAEC) are primary endothelial cells isolated from normal carotid arteries and are cryopreserved at third passage. HCtAEC can be propagated at least 10 doublings. The brachiocephalic artery is the first branch from the aortic arch. It bifurcates to form the right common carotid artery, which supplies blood to the head and neck, and the right subclavian artery, which supplies blood to the head and right arm. Both of the corresponding left arteries branch directly from the aortic arch. Carotid artery endothelial cells produce a chemotactic factor for monocytes, but not neutrophils, and this signal is increased after exposure of the endothelial cells to very low-density lipoprotein (b-VLDL) 1. Reduced blood flow during pregnancy is associated with hypotrophy of the endothelial and smooth muscle cells of the carotid artery in the guinea pig2. Oxidative stress suppresses the secretion of endothelin-1 by bovine carotid artery endothelial cells3. Insulin and insulin-like growth factor I stimulate migration and tube forming activity of bovine carotid artery endothelial cells in an angiogenesis in vitro model4, while high glucose-induced serum factor sulfhydryl oxidation, causes growth inhibition5. Matrix metalloproteinases (MMPs) have been implicated in the formation of these plaques in the brachiocephalic artery6, and the carotid artery.7

  1. Berliner, JA et al., Arteriosclerosis 6(3):254 (1986).
  2. Jovanovic, S & Jovanovic, A. Hum Reprod. 13(4):1074 (1998).
  3. Saito, T et al., J Cardiovasc Pharmacol. 31(S1):S345 (1998).
  4. Nadao-Hayashi, J et al., Atherosclerosis. 92(2-3):141 (1992).
  5. Nakao-Hayashi, J et al., Atherosclerosis. 97((1):89 (1992).
  6. Johnson, JL et al., Proc Natl Acad Sci USA. 102(43):15575 (2005).
  7. Abilleira, S et al., J Med Genet. 43(12):897 (2006).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human normal carotid artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HCtAEC (3014-05a), 500 ml of MesoEndo Cell Growth Medium (212-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Transfer Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HCtAEC, adult Each kit contains one ampoule of >500,000 cells, 500 ml of MesoEndo Cell Growth Medium (212-500), and a Subculture Reagent Kit (090K). 1 kit
1 ampoule 		3014K-05a
3014-05a 		$838
$695
Proliferating HCtAEC, adult Cultured in Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium at third passage. T-25 flask
T-75 flask
6 well plate
96 well plate 		3015-25a
3015-75a
3015-6Wa
3015-96Wa 		$695
$885
$885
$1005
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
MesoEndo Growth Medium Optimized and ready for use 500 ml 212-500 $102
MesoEndo Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 212K-500 $110
Endothelial Cell Basal Medium Add Growth Supplements before use 500 ml 210-500 $53
MesoEndo Growth Supplements Added to Basal Medium to create Growth Medium 30 ml 212-GS $58
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500ml
500 ml 		113-500
113PR-500 		$105
$110
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51