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Human Carotid Artery Smooth Muscle Cells: HCtASMC



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Human Carotid Artery Smooth Muscle Cells, HCtASMC

Human Carotid Artery Smooth Muscle Cells (HCtASMC) provide an in vitro system to explore in vivo findings. Apolipoprotein E (apoE) knockout mice serve as a model for atherosclerotic lesion development, as fibrous plaques commonly form in the brachiocephalic artery after 6 months of a high-fat diet, thus providing a small well-defined area in which to study the progressive loss of plaque stability and eventual rupture1,2,3. Reduced blood flow during pregnancy is associated with hypotrophy of the endothelial and smooth muscle cells of the carotid artery in the guinea pig4.

  1. Williams, H et al., Arterioscler Thromb Vasc Biol. 22(5):788 (2002).
  2. Rosenfeld, ME et al., Cur Atheroscler Rep. 4(3):238 (2002).
  3. Johnson, JL & Jackson, CL. Atherosclerosis. 154(2):399 (2001).
  4. Jovanovic, S & Jovanovic, A. Hum Reprod. 13(4):1074 (1998).

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human normal carotid artery
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HCtASMC (3014-05a), 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HCtASMC, adult Each kit contains one ampoule of >500,000 cells, 500 ml of Smooth Muscle Cell Growth Medium (311-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		3514K-05a
3514-05a 		$797
$659
Proliferating HCtASMC, adult Cultured and shipped at third passage in Smooth Muscle Cell Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		3515-25a
3515-75a
3515-6Wa
3515-96Wa 		$659
$849
$849
$969
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Smooth Muscle Cell Growth Medium Optimized and ready for use 500 ml 311-500 $98
Smooth Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500ml 311K-500 $105
Smooth Muscle Cell Basal Medium Add Growth Supplements before use 500 ml 310-500 $53
Smooth Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium 30 ml 311-GS $53
Smooth Muscle Cell Differentiation Medium    250 ml 311D-250 $68
Smooth Muscle Cell Growth Medium without Antibiotics All-in-one ready-to-use media 500 ml 311A-500 $105
Smooth Muscle Cell Growth Medium without bFGF All-in-one ready-to-use media 500 ml 311b-500 $105
Smooth Muscle Cell Growth Medium without FBS All-in-one ready-to-use media 500 ml 311F-500 $105
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51