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Human Lung Microvascular Endothelial Cells: HLMVEC



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A: Human Lung Microvascular Endothelial Cells, HLMVEC. B: HLMVEC immunofluorescently stained with anti-CD31 antibody (Cat# CB13678) and nucleus stained with PI. C&D: HLMVEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit.

The pulmonary vasculature is of great physiological/pathological significance.  It is now recognized to be a tissue composed of metabolically active, functionally responsive cells, that interact with circulating substrates and formed elements in ways that regulate the composition of systemic arterial blood, affect target organ functions, and contribute to thrombosis, hemostasis and immune reactions, as well as tumor metastasis1.
 
HLMVEC and elevated expression of chemoattractant cytokines are critical for directed migration of leucocytes into the lung during the induction and perpetuation of acute lung injury2. Human Lung Microvascular Endothelial Cells (HLMVEC) provide a useful tool for studying various aspects of pathology and biology of the pulmonary microvasculature in vitro.  
 
References:
1. Hewett, P.W.et al, Microvas. Res. 46:89 (1993)
2. McCuscey R, et al, Cardiovasc Res. 32:752 (1996)
 
Characterization:
  • Positive for factor VIII-related antigen expression.
  • Positive for CD31 expression.
  • Positive for metabolic activity by DiI-Ac-LDL uptake.
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human lung
Cryopreserved ampoule:
3rd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
An ampoule of cryopreserved HLMVEC (540-05), 500 ml of Microvascular Endothelial Cell Growth Medium (111-500), Attachment Factor Solution (123-100), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Transfer Media at 4th passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 15 doublings

Cytofect Endothelial Cell Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HLMVEC, adult Each kit contains an ampoule of cryopreserved HLMVEC (540-05), 500 ml of Microvascular Endothelial Cell Growth Medium (111-500), 100 ml of Attachment Factor Solution (123-100), a Subculture Reagent Kit. 1 Kit
1 Ampoule 		540K-05a
540-05a 		$877
$670
Proliferating HLMVEC, adult Cultured at fourth passage in Microvascular Endothelial Cell Growth Medium and shipped in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		541-25a
541-75a
541-6Wa
541-96Wa 		$670
$860
$860
$980
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Microvascular Endothelial Cell Growth Medium Optimized and ready for use 500 ml 111-500 $120
Microvascular Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 111K-500 $128
Microvascular Endothelial Cell Basal Medium Add Growth Supplement before use 500 ml 100-500 $53
Microvascular Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium 50 ml 111-GS $75
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
Endothelial Cell Starvation Medium Endothelial Cell Starvation Medium 250 ml
250 ml 		209-250
209PR-250 		$58
$68
Attachment Factor Solution Promotes attachment of endothelial cells. 100 ml 123-100 $18
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51