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Human Aortic Endothelial Cells: HAOEC



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A: Human Aortic Endothelial Cells, HAOEC.B: HAOEC immunofluorescently stained with Anti-CD31 (Cat# CB13678) and nucleus counterstained with PI. C&D: HAOEC transfected with GFP plasmid DNA using the Cytofect™ Endothelial Cell Transfection Kit.

Human Aortic Endothelial Cells (HAOEC) are primary endothelial cells isolated from normal human aorta. They are cryopreserved at second passage and can be cultured and propagated at least 16 population doublings. They respond to cytokine stimulation by expressing cell adhesion molecules. Aortic endothelial cells have been used as an in vitro model for the study of morphological and ultra-structural changes1 in the development of atherosclerosis. HAOEC system has also been used for the study of endothelial cell-dependent lymphocyte activation2 and chemoattractant activity for lymphocytes3 in relation to the degree of atherosclerosis.
  1. Antonor, A.S. et al, Atherosclerosis 59:1 (1986).
  2. Ashida, E.R. et al, J. Clin. Invest. 67:1490 (1981).
  3. Farber, H.W. et al, Amer. J. Physiol. 262 (4 parts): H1088  (1992)  
Characterization:   Positive for Factor VIII-related antigen expression.  Positive for Dil-Ac-LDL uptake.
 
 
Endothelial Cells play a pivotal role in a diverse range of physiological processes, such as angiogenesis and as the selective blood barrier; and pathophysiological processes, including arterial disease and cancer development. At Cell Applications, select lots of HAOEC, HUVEC, and HMVEC undergo an additional set of characterization to demonstrate stimulation-dependent angiogenesis and other endothelial cell signaling pathways. 
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Human aorta
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved HAOEC(304-05a), 500 ml of Endothelial Cell Growth Medium (211-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Transfer Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 16 doublings

Cytofect Endothelial Transfection Kit
Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HAOEC, adult Each kit contains an ampoule of >500,000 cells (304-05a), 500 ml of Endothelial Cell Growth Medium (211-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		304K-05a
304-05a 		$788
$649
Proliferating HAOEC, adult Cultured in Endothelial Cell Growth Medium and shipped at third passage in Endothelial Cell Transfer Medium. T-25 Flask
T-75 Flask
6 well
96 well 		305-25a
305-75a
305-6Wa
305-96Wa 		$649
$839
$839
$959
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Endothelial Cell Growth Medium Optimized and ready for use 500 ml 211-500 $98
Endothelial Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 211K-500 $105
Endothelial Cell Basal Medium Add Growth Supplement before use. 500 ml 210-500 $53
Endothelial Cell Growth Supplement Added to Basal Medium to create Growth Medium. 15 ml 211-GS $53
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
Endothelial Cell Serum-Free Defined Medium For maintaining the monolayer of endothelial cells at 100% confluent culture. Does not contain VEGF, FGF, PDGF. It is supplemented with defined components to allow metabolic and growth factor studies without interference of extraneous factors. Available without Phenol Red (113PR-500). 500 ml
500 ml 		113-500
113PR-500 		$105
$110
 
RNA
Product Name Product Description Size Cat. No. Price
HAOEC RNA , adult Total RNA prepared from HAOEC 10 ug
25 ug 		304-R10a
304-R25a 		$325
$650