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Human Skeletal Muscle Cells: HSkMC



(Enlarge Image)
Left: Human Skeletal Muscle Cells, HSkMC.
Right: Fused HSkMC immunofluorescently stained with anti-Desmin antibody and nucleus counterstained with PI.

 

Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of limbs. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings. Human Skeletal Muscle Cells can undergo differentiation to exhibit actin and myosin myofilaments. HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia1.   HSkMC also have the potential to act as a cardiac graft, mending damage to the heart2. Recently, cultured Human Skeletal Muscle cells have been used in micro gravity experiments to study the effects of low gravity environments on Human Skeletal Muscle3. 
  1. O’Sullivan, G.H. et al, Biochem Pharmacol 61(12):1479-1485 (2001) 
  2. El Oakley, R.M. et al, Ann. Thorac. Surg. 71(5):1724-1733 (2001) 
  3. Slentz, D.H. et al, In Vitro Cell. Dev. Biol. Anim. 37(3):148-156 (2001)
Characterization: Positive for sarcomere myosin.
 
Skeletal Muscle Cells play an instrumental role in the glucose metabolism and diabetes, thus select HSkMC lots undergo an additional set of characterization at Cell Applications, to demonstrate that Insulin & AMPK signaling is sensitive to insulin stimulation. Click on the link below to learn more.
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human limb skeletal muscle
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved (150-05f), 500 ml of HSkMC Growth Medium (151-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Transfer Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 15 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HSkMC, fetal Each kit contains one ampoule of >500,000 cells, 500 ml Growth Medium (151-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		150K-05f
150-05f 		$771
$620
Proliferating HSkMC, fetal Cultured at third passage in Skeletal Muscle Cell Growth Medium and shipped in Skeletal Muscle Cell Transfer Medium T-25 Flask
T-75 Flask
6 well
96 well 		151-25f
151-75f
151-6Wf
151-96Wf 		$620
$810
$810
$930
Cryopreserved HSkMC, adult Each kit contains one ampoule of >500,000 cells, 500 ml Growth Medium (151-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		150K-05a
150-05a 		$796
$645
Proliferating HSkMC, adult Cultured at third passage in Skeletal Muscle Cell Growth Medium and shipped in Skeletal Muscle Cell Transfer Medium T-25 Flask
T-75 Flask
6 well
96 well 		151-25a
151-75a
151-6Wa
151-96Wa 		$645
$835
$835
$955
 
Media and Reagrents
Product Name Product Description Size Cat. No. Price
Skeletal Muscle Cell Growth Medium Optimized and ready for use 500 ml 151-500 $110
Skeletal Muscle Cell Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 151K-500 $119
Skeletal Muscle Cell Basal Medium Add Growth Supplement before use 500 ml 150-500 $58
Skeletal Muscle Cell Growth Supplement Added to Basal Medium to create Growth Medium. 50 ml (2 parts) 151-GS $61
Skeletal Muscle Cell Differentiation Medium Promotes differentiation of Skeletal Muscle. 250 ml 151D-250 $58
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HSkMC RNA, fetal Human Fetal Skeletal Muscle Cell RNA 10 ug
25 ug 		150-R10f
150-R25f 		$325
$650
Differentiated HSkMC Total RNA, fetal Total RNA prepared from HSkMC Multinucleated Myotubes. 10 ug
25 ug 		150D-R10f
150D-R25f 		$380
$760