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Human Osteoblasts: HOb



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Left: Human Osteoblasts, HOb 
Right: von Kossa stained- Human Osteoblasts.

Human Osteoblasts, HOb, are isolated from normal human bone. They are cryopreserved at second passage and can be cultured and propagated for at least 10 population doublings. HOb sythesizes bone matrix. Matrix mineralization can be induced in the presence of ascorbic acid and B-Glycerophosphate. These cells are commonly used for micro- and macroscopic investigations of the skeletal system, such as the effects of inflammatory mediators,1 the effects of bone morphogenic proteins,2 the physiological control of bone remodeling,3 the regulation of bone metabolism,4 and the study of the chemicals associated with physical trauma.5 
  1. Frost, A. et al, Acta. Orthop. Scand. 70(5):497-503 (1999) 
  2. Takahashi, K., Nippon Yakyurigaku Zasshi. 116(4):232-240 (2000) 
  3. Cornish, J. et al Bone 17(4 Suppl):249S-254S (1995) 
  4. Malpe, R. et al, J. Bone Miner. Res. 12(3):423-430 (1997) 
  5. Reiff. D.A. et al, J. Trauma 50(3):433-438 (2001)  
Characterization: Positive for bone mineralization.
 
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human bone
Cryopreserved ampoule:
2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved Human Osteoblasts (406-05), 500 ml of Human Osteoblast Growth Medium (417-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Osteoblast Growth Medium at 3rd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HOb, adult Each kit contains one ampoule of >500,000 cells, 500 ml Growth Medium (417-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		406K-05a
406-05a 		$784
$635
Cryopreserved HOb, fetal Each kit contains one ampoule of >500,000 cells, 500 ml Growth Medium (417-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		406K-05f
406-05f 		$808
$659
Proliferating HOb, adult Cultured at third passage in Osteoblast Growth Medium and shipped in Osteoblast Transfer Medium. T-25 Flask
T-75 Flask
6 multiwell plate
96 multiwell plate 		407-25a
407-75a
407-6Wa
407-96Wa 		$635
$825
$825
$945
Proliferating HOb, fetal Cultured at third passage in Osteoblast Growth Medium and shipped in Osteoblast Transfer Medium. T-25 Flask
T-75 Flask
6 multiwell plate
96 multiwell plate 		407-25f
407-75f
407-6Wf
407-96Wf 		$659
$849
$849
$969
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Osteoblast Growth Medium Optimized and ready for use. 500 ml 417-500 $108
Osteoblast Growth Medium Kit Basal Medium & Growth Supplement packaged separately. Yields 500 ml 417K-500 $116
Osteoblast Basal Medium Add Growth Supplement before use. 500 ml 416-500 $58
Osteoblast Growth Supplement Added to Basal Medium to create Growth Medium 50 ml 417-GS $58
Osteoblast Differentiation Medium Osteoblast Differentiation Medium 250 ml 417D-250 $74
Osteoblast Growth Medium, without FBS All-in-one ready-to-use media. 500 ml 417F-500 $116
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51
 
RNA
Product Name Product Description Size Cat. No. Price
HOb RNA , fetal Total RNA prepared from HOb 10 ug
25 ug 		406-R10f
406-R25f 		$325
$650
Differentiated HOb RNA, fetal Total RNA prepared from Differentiated HOb 10 ug
25 ug 		406D-R10f
406D-R25f 		$380
$760
Stimulated HOb RNA, fetal Total RNA prepared from Stimulated HOb 10 ug
25 ug 		406S-R10f
406S-R25f 		$380
$760