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Rat Skeletal Muscle Cells (RSkMC) are isolated from the skeletal muscle of the rat limb. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings. Muscle diseases can occur in rats with clinical presentations resembling human disorders, and thus the rat system can be a valuable intermediate disease model1,2,3. ATP concentration and hypoxia have been studied in rat skeletal muscle4,5.
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Shelton, GD & Enqvall, E, Neuromuscul Disord. 15(2):127 (2005).
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Ytterberg, SR, Curr Opin Rheumatol. 3(6):934 (1991).
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Winand, NJ et al., Neuromuscul Disord. 4(5-6): 433 (1994).
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Li, J et al., J Appl Physiol 95(2):577 (2003).
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Nioka, S et al., Adv Exp Med Biol. 510:267 (2003).
Products are for research use only. They are not intended for human, animal, or diagnostic applications.
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Tissue:
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Normal skeletal muscle of the rat limb
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Cryopreserved ampoule:
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1st passage, >500,000 cells in Rat Skeletal Muscle Cell Basal Medium containing 10% FBS & 10% DMSO.
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Kit contains:
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Ampoule of cryopreserved RSkMCa (R150-05a) or RSkMCn (R150-05n), 500 ml of Rat Skeletal Muscle Cell Growth Medium (R151-500), and a Subculture Reagent Kit (090K).
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Proliferating Cells:
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Shipped in Rat Skeletal Muscle Cell Growth Medium at 2nd passage in either flasks or multiwell dishes.
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Population doublings:
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Can be cultured at least 10 doublings
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