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Re-Differentiated Human Chondrocytes: RD-HC



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Re-Differentiated Human Chondrocytes, RD-HC

Chondrocytes in monolayer culture lose their phenotypic characteristics very quickly and become de-differentiated to a fibroblast-like phenotype1,2,3. Cartilage-specific Type II Collagen expression cannot be detected in these de-differentiated chondrocytes4. Now, Cell Applications provides Re-Differentiated Chondrocytes by encapsulating chondrocytes in an alginate bead suspension. These Re-Differentiated Chondrocytes produce their phenotypic marker Aggrecan and Type II Collagen again14,5. Cells are shipped 10 days after encapsulation. Re-Differentiated Choncdocytes are expected 3 weeks after encapsulation and should retain their phenotypic characteristics for at least one week after complete re-differentiation. These cells have been used for the repair of cartilage defects6.
  1. Kato, Y. et al, J. Cell Physiol. 120:354 (1984)
  2. Jennings, S. D. et al, Exp. Cell Res. 145:415 (1983)
  3. Kuettner, K. E. et al, J. Cell Biol. 93:751 (1982)
  4. Benya, P. D. et al, Cell 30:215 (1982)
  5. Guo, J. et al, Connect. Tissue Res. 19:277 (1989)
  6. Grande, D. A. et al, Biomed. Mat. Res. 34:211 (1997)
Characterization:
  • Exhibit spherical morphology when encapsulated in alginate beads.
  • Positive for aggrecan mRNA and Collagen Type II mRNA.
Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human articular cartilage
Cryopreserved ampoule:
N/A
Kit contains:
N/A
Proliferating Cells:
 
Shipped in Chondrocyte Differentiation Medium in flasks.
Population doublings:
N/A

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Proliferating RD-HC, adult Encapsulated in alginate beads and shipped in Chondrocyte Differentiation Medium. T-25 Flask
T-75 Flask 		403RD-25a
403RD-75a 		$845
$1035
Proliferating RD-HC, fetal Encapsulated in alginate beads and shipped in Chondrocyte Differentiation Medium. T-25 Flask
T-75 Flask 		403RD-25f
403RD-75f 		$805
$995
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Chondrocyte Growth Medium Optimized and ready for use 500 ml 411-500 $95
Chondrocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 411K-500 $103
Chondrocyte Basal Medium Add Growth Supplement before use 500 ml 410-500 $50
Chondrocyte Growth Supplement Added to Basal Medium to create Growth Medium 50 ml 411-GS $53
Chondrocyte Differentiation Medium    250 ml 411D-250 $89
Depolarization Solution    50 ml 073-50 $28
 
RNA
Product Name Product Description Size Cat. No. Price
Re-Differentiated Chondrocyte RNA, adult Total RNA prepared from RD-HC 10 ul
25 ul 		402RD-R10a
402RD-R25a 		$480
$960
Re-Differentiated Chondrocyte RNA, fetal Total RNA prepared from RD-HC 10 ul
25 ul 		402RD-R10f
402RD-R25f 		$495
$960