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Human Chondrocytes: HC



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Human Chondrocytes, HC

Human Chondrocytes, HC,  are derived from normal human articular cartilage. They are cryopreserved at the second passage and can be cultured and propagated at least 10 population doublings. Chondrocytes produce and maintain the extracellular matrix of cartilage.1 Chondrocytes grow in serial monolayer culture lose their phenotypic markers and became de-differentiated2. This de-differentiation stage can be reversed by culturing them in semi-solid gel3,4. HC has served as useful model for studying chondrocyte differentiation and the effects of cytokines and growth factors on the suppression and maintenance of differentiated cartilage phenotype5.

  1. Muir, H., BioEssays. 17(12):1039 (1995).
  2. Blanco, F. J. et al, J. Immunol. 154:4018 (1995)
  3. Guo, J. et al, Connect. Tissue Res. 19:277 (1989)
  4. Benya, P. D. et al, Cell 30:215 (1982)
  5. Goldring, M. B. et al, Joint Cartilage Degradation:281 (1993)

Characterization: Positive for aggrecan after differentiation.

Each lot tested negative for HIV, Hepatitis B and Hepatitis C and negative for mycoplasma, bacteria, yeast, fungi.
Products are for research use only. They are not intended for human, animal, or diagnostic applications.

Tissue:
Normal human articular cartilage
Cryopreserved ampoule:
1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO.
Kit contains:
Ampoule of cryopreserved (402-05) Human Chondrocytes, 500 ml of Human Chondrocyte Growth Medium (411-500), and a Subculture Reagent Kit (090K).
Proliferating Cells:
 
Shipped in Chondrocyte Growth Medium at 2nd passage in either flasks or multiwell dishes.
Population doublings:
Can be cultured at least 10 doublings

Call 800-645-0848 or 858-453-0848 to place an order.
Cell Products
Product Name Product Description Size Cat. No. Price
Cryopreserved HC, adult Each ampoule contains >500,000 cryopreserved cells. Each kit contains one ampoule, 500 ml Growth Medium (411-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		402K-05a
402-05a 		$761
$625
Cryopreserved HC, fetal Each ampoule contains >500,000 cryopreserved cells. Each kit contains one ampoule, 500 ml Growth Medium (411-500), and a Subculture Reagent Kit (090K). 1 Kit
1 Ampoule 		402K-05f
402-05f 		$761
$625
Proliferating HC, adult Cultured and shipped at third passage in Chondrocyte Growth Medium. T-25 Flask
T-75 Flask
6 well
96 well 		403-25a
403-75a
403-6Wa
403-96Wa 		$625
$815
$815
$935
Proliferating HC, fetal Cultured and shipped at third passage in Chondrocyte Growth Medium. T-25 Flask
T-75 Flask
6 well
6 well 		403-25f *
403-75f *
403-6Wf *
403-96Wf * 		$625
$815
$815
$935
 
Media and Reagents
Product Name Product Description Size Cat. No. Price
Chondrocyte Growth Medium Optimized and ready for use 500 ml 411-500 $95
Chondrocyte Growth Medium Kit Basal Medium & Growth Supplement packaged separately Yields 500 ml 411K-500 $103
Chondrocyte Basal Medium Add Growth Supplement before use 500 ml 410-500 $50
Chondrocyte Growth Supplement Added to Basal Medium to create Growth Medium 50 ml 411-GS $53
Chondrocyte Differentiation Medium    250 ml 411D-250 $89
Subculture Reagent Kit 100 ml each of HBSS, Trypsin/EDTA & Trypsin Neutralizing Solution 100 ml 090K $51