Skeletal muscle cells are major components to form skeletal muscle. They participate not only in force generation for movement, postural support and heat production, but also in metabolic regulations and maintenance of homeostasis. Metabolic dysfunctions of skeletal muscle cells led to various disease developments. The cultured Human Skeletal Muscle Cells (HSkMC) are widely used for a diversity of biomedical studies ranged from cell signaling to drug discovery. HSkMC have been used in the study of genetic muscular diseases such as Malignant Hyperthermia1. HSkMC also have the potential to act as a cardiac graft, mending damage to the heart.2 Recently, cultured Human Skeletal Muscle Cells have been used in micro gravity experiments to study the effects of low gravity environments on human skeletal muscle.3 Since HSkMC are major target cells of insulin action, they are extensively used for diabetes studies.4
Our pre-screened Human Skeletal Muscle Cells (HSkMC) are isolated from the skeletal muscle of hamstrings. They retain morphological, biochemical, and metabolic characteristics of skeletal muscle. They are cryopreserved at second passage and can be cultured and propagated for at least 15 population doublings. Our pre-screened HSkMCs can undergo differentiation to exhibit actin and myosin myofilaments. We pre-screened our Human Skeletal Muscle Cells for major cell signaling and metabolic pathways and markers.
1. O’Sullivan, G.H. et al, Biochem. Pharmacol. 61(12):1479-1485 (2001)
2. El Oakley, R.M. et al, Ann. Thorac. Surg. 71(5):1724-1733 (2001)
3. Slentz, D.H. et al, In Vitro Cell. Dev. Biol. Anim. 37(3):148-156 (2001)
4. Virkamäki, A. et al, J. Clin. Invest. 103(7):931-943 (1999)
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